Scholarlink

<p>G-protein-coupled receptor-based fluorescent sensors are widely used to correlate neuromodulatory signaling with brain function. Although experiments in transfected cells often reveal selectivity for individual neurotransmitters, sensor specificity in the brain frequently remains uncertain. Using imaging in mouse brains, we find that norepinephrine and dopamine cross-activate the respective sensors. Non-specific activation occurred when innervation of the cross-reacting transmitter was high, and silencing of specific innervation was indispensable for interpreting sensor fluorescence.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Norepinephrine and dopamine sensor crosstalk depends on local innervation density

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年8月5日

查看全文
  • Ricardo C. López,
  • Natalie Noble,
  • Özge D. Özçete,
  • Leonardo Silenzi,
  • Xintong Cai,
  • Gillian E. Handy,
  • Jonathan W. Andersen,
  • Tommaso Patriarchi,
  • Yulong Li,
  • Pascal S. Kaeser

摘要

G-protein-coupled receptor-based fluorescent sensors are widely used to correlate neuromodulatory signaling with brain function. Although experiments in transfected cells often reveal selectivity for individual neurotransmitters, sensor specificity in the brain frequently remains uncertain. Using imaging in mouse brains, we find that norepinephrine and dopamine cross-activate the respective sensors. Non-specific activation occurred when innervation of the cross-reacting transmitter was high, and silencing of specific innervation was indispensable for interpreting sensor fluorescence.