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<p>Spontaneously blinking fluorophores toggle between nonfluorescent and fluorescent forms without caging groups or redox buffers, enabling super-resolution imaging. The intrinsic blinking of such dyes is governed by molecular structure and modulated by environment; there is no one-size-fits-all fluorophore suitable for every imaging context. We report dyes with tuned on:off ratios that enable single-molecule localization microscopy and super-resolution optical fluctuation imaging of biomolecular structures in vitro and in cells.</p>

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A series of spontaneously blinking dyes for super-resolution microscopy

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月15日

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  • Katie L. Holland,
  • Sarah E. Plutkis,
  • Brian P. English,
  • Timothy A. Daugird,
  • Abhishek Sau,
  • Jonathan B. Grimm,
  • Qinsi Zheng,
  • Ankith Sharma,
  • Sandeep Dave,
  • Anja Schmidt,
  • Fariha Rahman,
  • Liangqi Xie,
  • Peng Dong,
  • Ariana N. Tkachuk,
  • Timothy A. Brown,
  • Robert H. Singer,
  • Zhe J. Liu,
  • Siegfried M. Musser,
  • Wesley R. Legant,
  • Catherine G. Galbraith,
  • Luke D. Lavis

摘要

Spontaneously blinking fluorophores toggle between nonfluorescent and fluorescent forms without caging groups or redox buffers, enabling super-resolution imaging. The intrinsic blinking of such dyes is governed by molecular structure and modulated by environment; there is no one-size-fits-all fluorophore suitable for every imaging context. We report dyes with tuned on:off ratios that enable single-molecule localization microscopy and super-resolution optical fluctuation imaging of biomolecular structures in vitro and in cells.