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<p>Clubroot disease, caused by the obligate intracellular rhizarian protist <i>Plasmodiophora brassicae</i>, is devastating to cruciferous crops worldwide. Widespread field <i>P.</i> <i>brassicae</i> pathotypes frequently overcome the pathotype-specific resistance of modern varieties, posing a challenge for durable control of this disease. Here a genome-wide association study of 3 years of data comprising field clubroot phenotyping of 244 genome-resequenced <i>Brassica napus</i> accessions identified a strong association of <i>β-1,3-glucan synthase-like 5</i> (<i>GSL5</i>) with clubroot susceptibility. <i>GSL5</i> was evolutionarily conserved, and inactivation of <i>GSL5</i> by genome editing in <i>Arabidopsis</i>, <i>B.</i> <i>napus</i>, <i>Brassica</i> <i>rapa</i> and <i>Brassica oleracea</i> conferred broad-spectrum, high-level resistance to <i>P.</i> <i>brassicae</i> pathotypes without yield penalties in <i>B.</i> <i>napus</i>. <i>GSL5</i> inactivation derepressed the jasmonic acid-mediated immunity during <i>P.</i> <i>brassicae</i> secondary infection, and this immune repression was possibly reinforced through stabilization of GSL5 by a <i>P.</i> <i>brassicae</i> effector, facilitating clubroot susceptibility. Our study provides durable resistance resources for cruciferous clubroot disease control and insights into plant resistance against intracellular eukaryotic phytopathogens.</p>

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Inactivation of β-1,3-glucan synthase-like 5 confers broad-spectrum resistance to Plasmodiophora brassicae pathotypes in cruciferous plants

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年9月1日

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  • Yupo Wu,
  • Chuanji Zhao,
  • Yi Zhang,
  • Cuicui Shen,
  • Yuanyuan Zhang,
  • Xiong Zhang,
  • Lixia Gao,
  • Lingyi Zeng,
  • Qinglin Ke,
  • Li Qin,
  • Fan Liu,
  • Junyan Huang,
  • Li Ren,
  • Yueying Liu,
  • Hongtao Cheng,
  • Chaobo Tong,
  • Qiong Hu,
  • Xiaohui Cheng,
  • Yangdou Wei,
  • Shengyi Liu,
  • Lijiang Liu

摘要

Clubroot disease, caused by the obligate intracellular rhizarian protist Plasmodiophora brassicae, is devastating to cruciferous crops worldwide. Widespread field P. brassicae pathotypes frequently overcome the pathotype-specific resistance of modern varieties, posing a challenge for durable control of this disease. Here a genome-wide association study of 3 years of data comprising field clubroot phenotyping of 244 genome-resequenced Brassica napus accessions identified a strong association of β-1,3-glucan synthase-like 5 (GSL5) with clubroot susceptibility. GSL5 was evolutionarily conserved, and inactivation of GSL5 by genome editing in Arabidopsis, B. napus, Brassica rapa and Brassica oleracea conferred broad-spectrum, high-level resistance to P. brassicae pathotypes without yield penalties in B. napus. GSL5 inactivation derepressed the jasmonic acid-mediated immunity during P. brassicae secondary infection, and this immune repression was possibly reinforced through stabilization of GSL5 by a P. brassicae effector, facilitating clubroot susceptibility. Our study provides durable resistance resources for cruciferous clubroot disease control and insights into plant resistance against intracellular eukaryotic phytopathogens.