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<p>Resistance to wheat powdery mildew is commonly mediated by individual resistance proteins, most of which encode nucleotide-binding leucine-rich repeat (NLR) receptors. Here we report that the powdery mildew resistance gene <i>MLIW170</i>/<i>PM26</i> in wild emmer and bread wheat derivatives is determined by a genetically linked atypical NLR pair <i>TdCNL1</i>/<i>TdCNL5</i>. Map-based cloning and PacBio HiFi long-read sequencing revealed that <i>TdCNL1</i> encodes an atypical coiled-coil-domain-containing NLR protein (CNL) fused with a new potassium-dependent sodium-calcium exchanger integrated domain, whereas <i>TdCNL5</i> encodes a canonical CNL protein. Mutagenesis and virus-induced gene silencing experiments indicated that both <i>TdCNL1</i> and <i>TdCNL5</i> are essential for powdery mildew resistance. Transgenic plants with <i>TdCNL1</i> alone or <i>TdCNL1</i>/<i>TdCNL5</i> together show resistance, whereas Fielder with <i>TdCNL5</i> alone was susceptible. Geographically, <i>MLIW170</i>/<i>PM26</i> occurs in a few Southern populations of wild emmer wheat. Our study highlights an atypical NLR pair coordinately regulating powdery mildew resistance and provides a diversified resistance gene resource for wheat improvement.</p>

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An atypical NLR pair TdCNL1/TdCNL5 from wild emmer confers powdery mildew resistance in wheat

  • 期刊论文

  • 发表日期：

    2025年6月9日

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  • Keyu Zhu,
  • Miaomiao Li,
  • Lingli Dong,
  • Huaizhi Zhang,
  • Deyun Zhang,
  • Ping Lu,
  • Qiuhong Wu,
  • Jingzhong Xie,
  • Yongxing Chen,
  • Guanghao Guo,
  • Panpan Zhang,
  • Beibei Li,
  • Wenling Li,
  • Lei Dong,
  • Yikun Hou,
  • Yijun Yang,
  • Dan Qiu,
  • Gaojie Wang,
  • Baoge Huang,
  • Xuejia Cui,
  • Hongkui Fu,
  • Chengguo Yuan,
  • Tzion Fahima,
  • Eviatar Nevo,
  • Hongjie Li,
  • Junkang Rong,
  • Wei Hua,
  • Zhiyong Liu

摘要

Resistance to wheat powdery mildew is commonly mediated by individual resistance proteins, most of which encode nucleotide-binding leucine-rich repeat (NLR) receptors. Here we report that the powdery mildew resistance gene MLIW170/PM26 in wild emmer and bread wheat derivatives is determined by a genetically linked atypical NLR pair TdCNL1/TdCNL5. Map-based cloning and PacBio HiFi long-read sequencing revealed that TdCNL1 encodes an atypical coiled-coil-domain-containing NLR protein (CNL) fused with a new potassium-dependent sodium-calcium exchanger integrated domain, whereas TdCNL5 encodes a canonical CNL protein. Mutagenesis and virus-induced gene silencing experiments indicated that both TdCNL1 and TdCNL5 are essential for powdery mildew resistance. Transgenic plants with TdCNL1 alone or TdCNL1/TdCNL5 together show resistance, whereas Fielder with TdCNL5 alone was susceptible. Geographically, MLIW170/PM26 occurs in a few Southern populations of wild emmer wheat. Our study highlights an atypical NLR pair coordinately regulating powdery mildew resistance and provides a diversified resistance gene resource for wheat improvement.