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<p>One-carbon feedstocks such as formate could be promising renewable substrates for sustainable microbial production of food, fuels and chemicals. Here we replace the native energy-inefficient Calvin–Benson–Bassham cycle in <i>Cupriavidus necator</i> with the more energy-efficient reductive glycine pathway for growth on formate and CO<sub>2</sub>. In chemostats, our engineered strain reached a 17% higher biomass yield than the wild type and a yield higher than any natural formatotroph using the Calvin cycle. This shows the potential of synthetic metabolism to realize sustainable, bio-based production.</p>

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One-carbon fixation via the synthetic reductive glycine pathway exceeds yield of the Calvin cycle

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年2月27日

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  • Beau Dronsella,
  • Enrico Orsi,
  • Helena Schulz-Mirbach,
  • Sara Benito-Vaquerizo,
  • Suzan Yilmaz,
  • Timo Glatter,
  • Arren Bar-Even,
  • Tobias J. Erb,
  • Nico J. Claassens

摘要

One-carbon feedstocks such as formate could be promising renewable substrates for sustainable microbial production of food, fuels and chemicals. Here we replace the native energy-inefficient Calvin–Benson–Bassham cycle in Cupriavidus necator with the more energy-efficient reductive glycine pathway for growth on formate and CO2. In chemostats, our engineered strain reached a 17% higher biomass yield than the wild type and a yield higher than any natural formatotroph using the Calvin cycle. This shows the potential of synthetic metabolism to realize sustainable, bio-based production.