Scholarlink

<p>Biomolecular condensates composed of proteins and RNA are one approach by which cells regulate post-transcriptional gene expression. Their formation typically involves the phase separation of intrinsically disordered proteins with a target mRNA, sequestering the mRNA into a liquid condensate. This sequestration regulates gene expression by modulating translation or facilitating RNA processing. Here we engineer synthetic condensates using a fusion of an RNA-binding protein, the human Pumilio2 homology domain (Pum2), and a synthetic intrinsically disordered protein, an elastin-like polypeptide (ELP), that can bind and sequester a target mRNA transcript. In protocells, sequestration of a target mRNA largely limits its translation. Conversely, in <i>Escherichia coli</i>, sequestration of the same target mRNA increases its translation. We characterize the Pum2–ELP condensate system using microscopy, biophysical and biochemical assays, and RNA sequencing. This approach enables the modulation of cell function via the formation of synthetic biomolecular condensates that regulate the expression of a target protein.</p><p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Synthetic biomolecular condensates enhance translation from a target mRNA in living cells

  • 期刊论文

  • 发表日期：

    2025年2月10日

查看全文
  • Daniel Mark Shapiro,
  • Sonal Deshpande,
  • Seyed Ali Eghtesadi,
  • Miranda Zhong,
  • Cassio Mendes Fontes,
  • David Fiflis,
  • Dahlia Rohm,
  • Junseon Min,
  • Taranpreet Kaur,
  • Joanna Peng,
  • Max Ney,
  • Jonathan Su,
  • Yifan Dai,
  • Aravind Asokan,
  • Charles A. Gersbach,
  • Ashutosh Chilkoti

摘要

Biomolecular condensates composed of proteins and RNA are one approach by which cells regulate post-transcriptional gene expression. Their formation typically involves the phase separation of intrinsically disordered proteins with a target mRNA, sequestering the mRNA into a liquid condensate. This sequestration regulates gene expression by modulating translation or facilitating RNA processing. Here we engineer synthetic condensates using a fusion of an RNA-binding protein, the human Pumilio2 homology domain (Pum2), and a synthetic intrinsically disordered protein, an elastin-like polypeptide (ELP), that can bind and sequester a target mRNA transcript. In protocells, sequestration of a target mRNA largely limits its translation. Conversely, in Escherichia coli, sequestration of the same target mRNA increases its translation. We characterize the Pum2–ELP condensate system using microscopy, biophysical and biochemical assays, and RNA sequencing. This approach enables the modulation of cell function via the formation of synthetic biomolecular condensates that regulate the expression of a target protein.