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<p>A modified live attenuated African swine fever genotype II virus, GΔDKE-CmutQ96R/K108D, with deletions of three genes, <i>DP148R</i>, <i>EP153R,</i> and <i>K145R</i> and expressing a mutated CD2v protein with a non-haemadsorbing phenotype, was further modified by first removing two reporter gene cassettes expressing fluorescent proteins. The <i>B125R</i> gene was then deleted and one reporter cassette was reinserted as a marker. Groups of pigs were immunised with this virus using a range of doses from 100 to 10,000 infectious particles. One pig immunised with the lowest dose reached a moderate severity humane endpoint. The other pigs showed mild or no clinical signs. Low levels of the virus used for immunisation were detected post-immunisation. After challenge with virulent virus, all pigs were protected, and few clinical signs were observed. Low levels of replication of the challenge virus were detected in seven from the twenty-three challenged pigs and no virus in the remaining pigs.</p>

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Deletion of B125R increases protection induced by a genotype II African swine fever vaccine candidate

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年3月19日

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  • Anusyah Rathakrishnan,
  • Ana Luisa Reis,
  • Katy Moffat,
  • Lynnette Goatley,
  • Elisenda Viaplana,
  • Jose Carlos Mancera,
  • Alicia Urniza,
  • Linda K. Dixon

摘要

A modified live attenuated African swine fever genotype II virus, GΔDKE-CmutQ96R/K108D, with deletions of three genes, DP148R, EP153R, and K145R and expressing a mutated CD2v protein with a non-haemadsorbing phenotype, was further modified by first removing two reporter gene cassettes expressing fluorescent proteins. The B125R gene was then deleted and one reporter cassette was reinserted as a marker. Groups of pigs were immunised with this virus using a range of doses from 100 to 10,000 infectious particles. One pig immunised with the lowest dose reached a moderate severity humane endpoint. The other pigs showed mild or no clinical signs. Low levels of the virus used for immunisation were detected post-immunisation. After challenge with virulent virus, all pigs were protected, and few clinical signs were observed. Low levels of replication of the challenge virus were detected in seven from the twenty-three challenged pigs and no virus in the remaining pigs.