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<p>Lineage tracing remains an essential approach towards understanding cell fate, tissue formation, and human development. Herein, we review advancements in lineage tracing techniques, the integration of sequencing and imaging technologies, and computational tools for analysis. We report on recent lineage tracing applications, including integrative techniques (MADM-CloneSeq), in situ hybridization (DART-FISH), and modern workflows (BaSISS), which hold an essential role in our aim to unravel lineage hierarchies and, ultimately, human development.</p>

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Next generation lineage tracing and its applications to unravel development

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年6月5日

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  • Spencer Short,
  • Rodrigo García-Tejera,
  • Linus J. Schumacher,
  • Daniel L. Coutu

摘要

Lineage tracing remains an essential approach towards understanding cell fate, tissue formation, and human development. Herein, we review advancements in lineage tracing techniques, the integration of sequencing and imaging technologies, and computational tools for analysis. We report on recent lineage tracing applications, including integrative techniques (MADM-CloneSeq), in situ hybridization (DART-FISH), and modern workflows (BaSISS), which hold an essential role in our aim to unravel lineage hierarchies and, ultimately, human development.