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<p>Antimicrobial resistance in mastitis-associated pathogens has heightened concerns over the safety of raw milk, which transmits pathogens from animals to humans. Elevated somatic cell count (SCC) is widely recognized as a key indicator of microbial quality in milk, often linked to mastitis infections. High SCC alters milk composition, compromises milk quality, and reduces shelf life, rendering it unsuitable for consumption. Current methods for quantitative SSC, such as flow cytometry and microscopy, are effective but impractical for on-site applications. To bridge this gap, we developed QuantM, a portable and affordable device that employs amine-functionalized iron oxide nanoparticles to isolate somatic cells from milk via surfactant-induced aggregation. It quantifies SCC in field conditions within 10 minutes, achieving 94.74% sensitivity, 90.0% specificity, and 94.74% accuracy, with a detection limit of 0.017 million cells/mL compared to flow cytometry. This low-cost solution (&lt;$0.10 per test) promises to enhance milk quality and safeguard public health.</p>

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Portable and affordable device for on-site quantitative detection of somatic cells in milk

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年8月22日

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  • Yathirajarao Tammineni,
  • Pankaj Kumar,
  • Shashidhar Reddy,
  • Muskan,
  • Komal Birader,
  • Kapil Kumar,
  • Anitha Arumugam,
  • Sujit Saha,
  • Nilesh Nayee,
  • Pankaj Suman

摘要

Antimicrobial resistance in mastitis-associated pathogens has heightened concerns over the safety of raw milk, which transmits pathogens from animals to humans. Elevated somatic cell count (SCC) is widely recognized as a key indicator of microbial quality in milk, often linked to mastitis infections. High SCC alters milk composition, compromises milk quality, and reduces shelf life, rendering it unsuitable for consumption. Current methods for quantitative SSC, such as flow cytometry and microscopy, are effective but impractical for on-site applications. To bridge this gap, we developed QuantM, a portable and affordable device that employs amine-functionalized iron oxide nanoparticles to isolate somatic cells from milk via surfactant-induced aggregation. It quantifies SCC in field conditions within 10 minutes, achieving 94.74% sensitivity, 90.0% specificity, and 94.74% accuracy, with a detection limit of 0.017 million cells/mL compared to flow cytometry. This low-cost solution (<$0.10 per test) promises to enhance milk quality and safeguard public health.