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<p>Treatment with C18:1 and C18:2, but not C18:0, increased the triterpenoid content of the medicinal fungus <i>Sanghuangporus lonicericola</i>. We identified 413 terpenoids, including 210 volatile terpenoids. Eight upregulated terpenoids, including 3,13,15-trihydroxyoleanane-12-one, dulcioic acid and serrat-14-ene-3,20,24,29-tetrol, were shared between the C18:1 and C18:2 treatments but not the C18:0 treatment. The C18:1 and C18:2 treatments increased the levels of 12 and 7 odour-related terpenoids, respectively, and increased the level of alpha-farnesene (herbal odour). Gene set enrichment analysis revealed that compared with C18:0, C18:1 and C18:2 produced stronger activation of the terpenoid biosynthesis, fatty acid degradation, and MAPK signalling pathways and stronger inhibition of basal transcription factors at both the transcript and protein levels. Finally, two-way orthogonal partial least squares analysis revealed that gene and protein expression in the identified pathways was correlated with levels of unsaturated fatty acid-induced terpenoid metabolites. Together, our integrated multiomics data revealed the key pathways involved in unsaturated fatty acid-induced terpenoid biosynthesis in <i>S. lonicericola</i>.</p>

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Multiomics reveals the molecular mechanism of unsaturated fatty acid-induced terpenoid biosynthesis in Sanghuangporus lonicericola

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年3月26日

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  • Lu Guo,
  • Yuan-Gen Liu,
  • Ying-Wen Fu,
  • Yu-Yan Wang,
  • Hao-Jin Wang,
  • Shu-Mei Zhu,
  • Qi-Zhi He,
  • Dong-Xue Zhang,
  • Shan-Shan Zhu,
  • Si-Xian Wang,
  • Tian Tong,
  • Xu-Jie Dong,
  • Xiao-Ling Wang,
  • Yong-Nan Liu,
  • Gao-Qiang Liu

摘要

Treatment with C18:1 and C18:2, but not C18:0, increased the triterpenoid content of the medicinal fungus Sanghuangporus lonicericola. We identified 413 terpenoids, including 210 volatile terpenoids. Eight upregulated terpenoids, including 3,13,15-trihydroxyoleanane-12-one, dulcioic acid and serrat-14-ene-3,20,24,29-tetrol, were shared between the C18:1 and C18:2 treatments but not the C18:0 treatment. The C18:1 and C18:2 treatments increased the levels of 12 and 7 odour-related terpenoids, respectively, and increased the level of alpha-farnesene (herbal odour). Gene set enrichment analysis revealed that compared with C18:0, C18:1 and C18:2 produced stronger activation of the terpenoid biosynthesis, fatty acid degradation, and MAPK signalling pathways and stronger inhibition of basal transcription factors at both the transcript and protein levels. Finally, two-way orthogonal partial least squares analysis revealed that gene and protein expression in the identified pathways was correlated with levels of unsaturated fatty acid-induced terpenoid metabolites. Together, our integrated multiomics data revealed the key pathways involved in unsaturated fatty acid-induced terpenoid biosynthesis in S. lonicericola.