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<p>The demonstration of MJFR14-6-4-2 proximity ligation assay positive aggregates in brains affected by Parkinson’s disease and LRRK2 mutations, motivated the analysis of α-synuclein oligomers in brain cytosol from dementia with Lewy bodies (DLB) and control cases by combining size-exclusion chromatography, aggregate-specific MJFR14-6-4-2 ELISA and SDS-denaturing immunoblotting. We demonstrate a DLB-specific accumulation of large SDS-soluble oligomers, and the presence of medium-sized oligomers consisting of SDS-resistant α-synuclein dimers and trimers in both DLB and controls.</p>

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Higher-molecular-weight a-synuclein oligomers are increased in the brain cytosol of patients with dementia with Lewy bodies

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月28日

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  • Emil Gregersen,
  • Mia R. Antorini,
  • Lasse Reimer,
  • Ludovica Zaccagnini,
  • Dennis Selkoe,
  • Tim Bartels,
  • Poul Henning Jensen

摘要

The demonstration of MJFR14-6-4-2 proximity ligation assay positive aggregates in brains affected by Parkinson’s disease and LRRK2 mutations, motivated the analysis of α-synuclein oligomers in brain cytosol from dementia with Lewy bodies (DLB) and control cases by combining size-exclusion chromatography, aggregate-specific MJFR14-6-4-2 ELISA and SDS-denaturing immunoblotting. We demonstrate a DLB-specific accumulation of large SDS-soluble oligomers, and the presence of medium-sized oligomers consisting of SDS-resistant α-synuclein dimers and trimers in both DLB and controls.