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<p>Ageing is the primary risk factor for Parkinson’s disease, yet the intricate interplay between these processes remains ambiguous. This position paper, a collaborative output from the PD-AGE consortium, addresses the urgent need for standardising methods in in vitro modelling. A panel of international experts recommends human induced pluripotent stem cell (iPSC)-derived models, with chemically induced ageing methods, such as the SLO cocktail, as a robust system. Furthermore, the consortium highlights the value of direct and semi-direct reprogramming for retaining donor-specific ageing phenotypes. The paper also outlines a prioritised panel of measurable parameters, categorised into senescence, inflammaging, omics profiling, and mitochondrial dysfunction, providing a consistent framework to enhance research reproducibility, investigating the nexus of ageing and Parkinson’s. In addition, we provide links to SOPs (<a href="https://doi.org/10.5281/zenodo.15056603">https://doi.org/10.5281/zenodo.15056603</a>) [1] to measure the key measurable ageing parameters outlined in this review to facilitate consistency and reproducibility within the field.</p>

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Investigating the ageing-Parkinson’s disease nexus: standardisation of in vitro models and techniques by the PD-AGE network

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年10月9日

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  • Alexander G. Bury,
  • Alicja Olejnik,
  • Chiara Tocco,
  • Nathalie Saurat,
  • Elezabeth Stephen,
  • Dirk Hockemeyer,
  • Jens C. Schwamborn,
  • Lorenz Studer,
  • Pier Giorgio Mastroberardino,
  • Silvia Bolognin,
  • Tilo Kunath,
  • Viktor I. Korolchuk,
  • Janelle Drouin-Ouellet,
  • Heather Mortiboys

摘要

Ageing is the primary risk factor for Parkinson’s disease, yet the intricate interplay between these processes remains ambiguous. This position paper, a collaborative output from the PD-AGE consortium, addresses the urgent need for standardising methods in in vitro modelling. A panel of international experts recommends human induced pluripotent stem cell (iPSC)-derived models, with chemically induced ageing methods, such as the SLO cocktail, as a robust system. Furthermore, the consortium highlights the value of direct and semi-direct reprogramming for retaining donor-specific ageing phenotypes. The paper also outlines a prioritised panel of measurable parameters, categorised into senescence, inflammaging, omics profiling, and mitochondrial dysfunction, providing a consistent framework to enhance research reproducibility, investigating the nexus of ageing and Parkinson’s. In addition, we provide links to SOPs (https://doi.org/10.5281/zenodo.15056603) [1] to measure the key measurable ageing parameters outlined in this review to facilitate consistency and reproducibility within the field.