Scholarlink

<p>Studying neuronal cells in space reveals how microgravity affects brain function, gene expression, and cellular processes. This study details the preparation and validation of a 3D neuronal electrophysiology (EPHYS) sensing microfluidic biodevice used during a suborbital space flight. Initially, the device’s function was tested with rat hippocampal neurons using EPHYS data collected via a microelectrode array (MEA). This system was later applied to human glutamatergic (Glu) neurons for eight days preceding a suborbital flight. A live-dead assay confirmed cell viability, and the system was integrated into a CubeLab to maintain a controlled environment. Two biological samples were flown, along with two control samples, to validate the EPHYS system. Results showed that human Glu-neurons exposed to microgravity exhibited altered expression of vesicular glutamate transporters (VGLUTs) while maintaining neuronal differentiation markers. The findings contribute to understanding neurological disorders, neuro-inflammation, and cognitive impacts of space travel, with broader applications for brain health research on Earth.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Adoption of microfluidic MEA technology for electrophysiology of 3D neuronal networks exposed to suborbital conditions

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年5月27日

查看全文
  • Andie E. Padilla,
  • Gobinath C,
  • Candice Hovell,
  • Jeremy Mares,
  • Veerle Reumers,
  • Twyman Clements,
  • Jason Rextroat,
  • Paul Gamble,
  • Ben Lumpp,
  • Binata Joddar

摘要

Studying neuronal cells in space reveals how microgravity affects brain function, gene expression, and cellular processes. This study details the preparation and validation of a 3D neuronal electrophysiology (EPHYS) sensing microfluidic biodevice used during a suborbital space flight. Initially, the device’s function was tested with rat hippocampal neurons using EPHYS data collected via a microelectrode array (MEA). This system was later applied to human glutamatergic (Glu) neurons for eight days preceding a suborbital flight. A live-dead assay confirmed cell viability, and the system was integrated into a CubeLab to maintain a controlled environment. Two biological samples were flown, along with two control samples, to validate the EPHYS system. Results showed that human Glu-neurons exposed to microgravity exhibited altered expression of vesicular glutamate transporters (VGLUTs) while maintaining neuronal differentiation markers. The findings contribute to understanding neurological disorders, neuro-inflammation, and cognitive impacts of space travel, with broader applications for brain health research on Earth.