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<p>Global nitrogen (N) deposition, a major consequence of climate change, has profound impacts on soil microbes, yet comparative studies investigating the effects of different N types and levels on diverse soil microorganisms and their ecological functions remain scarce. Here, we conducted a 7-year simulated N deposition with multiple levels (Low, Medium, High) and multiple forms (NH<sub>4</sub>NO<sub>3</sub>, NH<sub>4</sub>Cl, KNO<sub>3</sub>), combining amplicon sequencing, QMEC chips and ¹⁵N isotope tracing to analyze their impacts on soil microbial communities of prokaryotes, fungi and Cercozoa, elemental cycling, and N transformation. The results showed that N forms dominated Cercozoa and prokaryote community structures (<i>P</i> &lt; 0.05) but not fungi. High NH₄⁺-N deposition significantly reduced microbial resistance (<i>P</i> &lt; 0.05), while NO₃<sup>-</sup>-N deposition enhanced prokaryote-Cercozoa bipartite network stability and linearly increased community resistance (<i>P</i> &lt; 0.05), supporting the ecological phenomenon of “Interdependence Leading to Enhanced Resilience”. Elevated N deposition increased microbial diversity but inhibited key C / N cycling genes (e.g., <i>pmoA</i>, <i>hzsB</i>, <i>nirK2</i>, and <i>nirS1</i>) and N transformation (<i>P</i> &lt; 0.05), raising CH₄ emission and soil N enrichment risks. In conclusion, this study provides scientific support for mitigating N deposition impacts and advancing environmental sustainability.</p>

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Heterogeneous responses of soil microbial communities and functions in coastal wetlands to long-term nitrogen deposition

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年3月17日

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  • Mengyue Sun,
  • Mingcong Li,
  • Baohua Xie,
  • Guangshan Wei,
  • Yuqi Zhou,
  • Wenxi Zhou,
  • Wenchong Shi,
  • Guangxuan Han,
  • Ye Deng,
  • Zheng Gao

摘要

Global nitrogen (N) deposition, a major consequence of climate change, has profound impacts on soil microbes, yet comparative studies investigating the effects of different N types and levels on diverse soil microorganisms and their ecological functions remain scarce. Here, we conducted a 7-year simulated N deposition with multiple levels (Low, Medium, High) and multiple forms (NH4NO3, NH4Cl, KNO3), combining amplicon sequencing, QMEC chips and ¹⁵N isotope tracing to analyze their impacts on soil microbial communities of prokaryotes, fungi and Cercozoa, elemental cycling, and N transformation. The results showed that N forms dominated Cercozoa and prokaryote community structures (P < 0.05) but not fungi. High NH₄⁺-N deposition significantly reduced microbial resistance (P < 0.05), while NO₃--N deposition enhanced prokaryote-Cercozoa bipartite network stability and linearly increased community resistance (P < 0.05), supporting the ecological phenomenon of “Interdependence Leading to Enhanced Resilience”. Elevated N deposition increased microbial diversity but inhibited key C / N cycling genes (e.g., pmoA, hzsB, nirK2, and nirS1) and N transformation (P < 0.05), raising CH₄ emission and soil N enrichment risks. In conclusion, this study provides scientific support for mitigating N deposition impacts and advancing environmental sustainability.