<p>Calcium homeostasis is tightly regulated due to the essential roles of calcium ions (Ca²⁺) in various cellular processes. CAX1 in <i>Arabidopsis thaliana</i> (AtCAX1) serves as a Ca²⁺/H⁺ exchanger transporting excess cytosolic Ca²⁺ into the vacuole, which is modulated by kinase phosphorylation in response to diverse signals. However, the regulatory mechanism remains unclear. Here we present the structures of wild-type AtCAX1 in an inactivated state and a phosphomimetic mutant in an activated state. In the wild-type structure, the amino-terminal region forms an α-helix that blocks the transport tunnel, thus inhibiting its transport activity. In contrast, in the phosphomimetic mutant structure, this blocking helix is released from the tunnel, leading to AtCAX1 activation. Conformational changes are also observed in the transmembrane domain. Together, these findings provide insights into the transport mechanism of the Ca²⁺/H⁺ exchangers and set up a basis for future studies of the regulation of calcium homeostasis in plants.</p>