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<p>Photorespiration is the second largest carbon flux in most leaves and is integrated into metabolism broadly including one-carbon (C<sub>1</sub>) metabolism. Photorespiratory intermediates such as serine and others may serve as sources of C<sub>1</sub> units, but it is unclear to what degree this happens in vivo, whether altered photorespiration changes flux to C<sub>1</sub> metabolism, and if so through which intermediates. To clarify these questions, we quantified carbon flux from photorespiration to C<sub>1</sub> metabolism using <sup>13</sup>CO<sub>2</sub> labelling and isotopically non-stationary metabolic flux analysis in <i>Arabidopsis thaliana</i> under different O<sub>2</sub> concentrations which modulate photorespiration. The results revealed that ~5.8% of assimilated carbon passes to C<sub>1</sub> metabolism under ambient photorespiratory conditions, but this flux greatly decreases under limited photorespiration. Furthermore, the primary carbon flux from photorespiration to C<sub>1</sub> metabolism is through serine. Our results provide fundamental insight into how photorespiration is integrated into C<sub>1</sub> metabolism, with possible implications for C<sub>1</sub> metabolic response to climate change.</p>

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Metabolic flux analysis in leaf metabolism quantifies the link between photorespiration and one carbon metabolism

  • 期刊论文

  • 发表日期：

    2025年9月3日

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  • Kelem Gashu,
  • Joshua A. M. Kaste,
  • Sanja Roje,
  • Berkley J. Walker

摘要

Photorespiration is the second largest carbon flux in most leaves and is integrated into metabolism broadly including one-carbon (C1) metabolism. Photorespiratory intermediates such as serine and others may serve as sources of C1 units, but it is unclear to what degree this happens in vivo, whether altered photorespiration changes flux to C1 metabolism, and if so through which intermediates. To clarify these questions, we quantified carbon flux from photorespiration to C1 metabolism using 13CO2 labelling and isotopically non-stationary metabolic flux analysis in Arabidopsis thaliana under different O2 concentrations which modulate photorespiration. The results revealed that ~5.8% of assimilated carbon passes to C1 metabolism under ambient photorespiratory conditions, but this flux greatly decreases under limited photorespiration. Furthermore, the primary carbon flux from photorespiration to C1 metabolism is through serine. Our results provide fundamental insight into how photorespiration is integrated into C1 metabolism, with possible implications for C1 metabolic response to climate change.