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<p><i>N</i><sup>6</sup>-Methyladenosine (m<sup>6</sup>A) reader proteins, which recognize m<sup>6</sup>A to regulate RNA metabolism, are important for plant adaptation to the changing environment. It remains unknown how the activities of plant m<sup>6</sup>A reader proteins are regulated in plant responses to stress. Here we show that the rice m<sup>6</sup>A reader protein EVOLUTIONARILY CONSERVED C-TERMINAL REGION 3 (OsECT3), required for rice tolerance to cold, is post-translationally modified by lysine acetylation, which reduces its m<sup>6</sup>A-binding activity. Under cold conditions, OsECT3 acetylation is reduced by cold-induced histone deacetylase HDA705 and low ACLA2-sourced acetyl-CoA levels, resulting in an increase in OsECT3 m<sup>6</sup>A-binding activity, the accumulation of cold-response-related mRNAs and improved tolerance of rice to cold stress. These results unravel a regulatory mechanism of an m<sup>6</sup>A reader protein to dynamically control m<sup>6</sup>A RNA levels under stress and suggest a link between lysine acetylation, metabolism and m<sup>6</sup>A pathways.</p>

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Regulation of m6A RNA reader protein OsECT3 activity by lysine acetylation in the cold stress response in rice

  • 期刊论文

  • 发表日期：

    2025年6月20日

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  • Nini Ma,
  • Peizhe Song,
  • Ziyang Liu,
  • Yangjie Li,
  • Zhihe Cai,
  • Mengyue Ding,
  • Xuan Ma,
  • Qiutao Xu,
  • Yaping Yue,
  • Tangdi Luo,
  • Dao-Xiu Zhou,
  • Guifang Jia,
  • Yu Zhao

摘要

N6-Methyladenosine (m6A) reader proteins, which recognize m6A to regulate RNA metabolism, are important for plant adaptation to the changing environment. It remains unknown how the activities of plant m6A reader proteins are regulated in plant responses to stress. Here we show that the rice m6A reader protein EVOLUTIONARILY CONSERVED C-TERMINAL REGION 3 (OsECT3), required for rice tolerance to cold, is post-translationally modified by lysine acetylation, which reduces its m6A-binding activity. Under cold conditions, OsECT3 acetylation is reduced by cold-induced histone deacetylase HDA705 and low ACLA2-sourced acetyl-CoA levels, resulting in an increase in OsECT3 m6A-binding activity, the accumulation of cold-response-related mRNAs and improved tolerance of rice to cold stress. These results unravel a regulatory mechanism of an m6A reader protein to dynamically control m6A RNA levels under stress and suggest a link between lysine acetylation, metabolism and m6A pathways.