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<p>Timely and sensitive detection of inflammatory and infectious lung diseases remains a major clinical challenge. Current diagnostic approaches often identify pathology only at advanced stages, leaving gaps in acute triage, fibrotic activity assessment, transplant rejection monitoring and inflammation follow-up. Magnetic Particle Imaging (MPI) offers a sensitive, background-free, whole-body imaging modality without ionizing radiation. Here, we present immuno-Magnetic Particle Imaging (immuno-MPI) using micron-sized iron oxide particles targeted to VCAM-1, a key marker of endothelial inflammation. In three lung inflammation models in male mice, MPIO@αVCAM-1 generates high-contrast images within 3 minutes following intravenous injection at 1 mg/kg, with rapid accumulation at the inflamed endothelium and fast clearance of unbound particles. Predominantly confined to the vasculature, these particles selectively target VCAM-1 and discriminate inflamed from healthy lungs at doses as low as 0.18 mg/kg (7.5 µg iron). Specificity for VCAM-1 is confirmed by the absence of signal using control antibodies (MPIO@IgG) and competitive blocking with free αVCAM-1 antibodies. Overall, immuno-MPI provides a non-invasive approach that avoids ionizing radiation and supports pulmonary inflammation monitoring.</p>

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Ultrasensitive detection of lung inflammation in male mice using immuno-magnetic particle imaging

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月23日

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  • Swannie Pedron,
  • Sara Martinez de Lizarrondo,
  • Laurent D’Arco,
  • Jolan Malherbe,
  • Sophie Trébel,
  • Jurie Tashkandi,
  • Karen Alt,
  • Bertrand Galy,
  • Pierre-Simon Bellaye,
  • Jonathan Vigne,
  • Denis Vivien,
  • Maxime Gauberti,
  • Thomas Bonnard

摘要

Timely and sensitive detection of inflammatory and infectious lung diseases remains a major clinical challenge. Current diagnostic approaches often identify pathology only at advanced stages, leaving gaps in acute triage, fibrotic activity assessment, transplant rejection monitoring and inflammation follow-up. Magnetic Particle Imaging (MPI) offers a sensitive, background-free, whole-body imaging modality without ionizing radiation. Here, we present immuno-Magnetic Particle Imaging (immuno-MPI) using micron-sized iron oxide particles targeted to VCAM-1, a key marker of endothelial inflammation. In three lung inflammation models in male mice, MPIO@αVCAM-1 generates high-contrast images within 3 minutes following intravenous injection at 1 mg/kg, with rapid accumulation at the inflamed endothelium and fast clearance of unbound particles. Predominantly confined to the vasculature, these particles selectively target VCAM-1 and discriminate inflamed from healthy lungs at doses as low as 0.18 mg/kg (7.5 µg iron). Specificity for VCAM-1 is confirmed by the absence of signal using control antibodies (MPIO@IgG) and competitive blocking with free αVCAM-1 antibodies. Overall, immuno-MPI provides a non-invasive approach that avoids ionizing radiation and supports pulmonary inflammation monitoring.