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<p>Aqueous two-phase system (ATPS) droplets in cells act as fluidic microreactors by concentrating biomacromolecules. Inspired by this phenomenon, dextran-rich microdroplets formed in an ATPS with polyethylene glycol have been explored as artificial microreactors for sensitive detection and spatiotemporal control of biochemical reactions. However, the rapid fusion of the microdroplets into bulk phase separation has limited practical applications of this approach. Here, we report the stabilization of dextran-rich microdroplets using supramolecular nanofibers of an azobenzene-appending self-assembling peptide (AzSAP). Physicochemical characterization and structural analyses elucidate the mechanism of nanofiber formation and its role in droplet stabilization. The nanofiber network prevents droplet coalescence while maintaining macroscopic fluidity, thereby enabling highly sensitive quantitative virus detection via microfluidic analysis by confining infecting viruses within droplets. Furthermore, the photo-responsive properties of the AzSAP allow dynamic control over droplet size and intra-droplet virus activity, highlighting its exceptional potential as a platform for programmable artificial microreactors.</p>

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Supramolecular nanofiber stabilization of microdroplets enables ultra-sensitive virus infection analysis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月22日

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  • Noriyuki Uchida,
  • Atsuya Yaguchi,
  • Shiori Kondo,
  • Kazuyoshi Muranishi,
  • Haruka Kawabata,
  • Haruka Umezawa,
  • Naito Ishimoto,
  • Michiko Tajiri,
  • Satoko Akashi,
  • Takuya Seki,
  • Go Watanabe,
  • Ayano Yoshida,
  • Genki Higuchi,
  • Jakuei Fu,
  • Takanobu Takenouchi,
  • Daisuke Yoshino,
  • Hirotsugu Hiramatsu,
  • Masaki Okumura,
  • Tomohide Saio,
  • Hiroyuki Noji,
  • Young-Ho Lee,
  • Sam-Yong Park,
  • Takahiro Muraoka

摘要

Aqueous two-phase system (ATPS) droplets in cells act as fluidic microreactors by concentrating biomacromolecules. Inspired by this phenomenon, dextran-rich microdroplets formed in an ATPS with polyethylene glycol have been explored as artificial microreactors for sensitive detection and spatiotemporal control of biochemical reactions. However, the rapid fusion of the microdroplets into bulk phase separation has limited practical applications of this approach. Here, we report the stabilization of dextran-rich microdroplets using supramolecular nanofibers of an azobenzene-appending self-assembling peptide (AzSAP). Physicochemical characterization and structural analyses elucidate the mechanism of nanofiber formation and its role in droplet stabilization. The nanofiber network prevents droplet coalescence while maintaining macroscopic fluidity, thereby enabling highly sensitive quantitative virus detection via microfluidic analysis by confining infecting viruses within droplets. Furthermore, the photo-responsive properties of the AzSAP allow dynamic control over droplet size and intra-droplet virus activity, highlighting its exceptional potential as a platform for programmable artificial microreactors.