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<p>The endosperm is the predominant storage organ for starch and storage proteins in cereal grains, which also determines both grain yield and quality. Although key enzymes involved in the biosynthesis of starch and storage proteins have been identified, the underlying regulatory mechanisms remain largely obscure. In this study, we identified OsMYB4, a MYB transcription factor, specifically expressed in rice (<i>Oryza sativa</i> L.) endosperm. Loss-of-function of <i>OsMYB4</i> led to increased grain chalkiness, accompanied by reduced amylose and protein contents as well as decreased grain yield. OsMYB4 directly binds to the promoters of multiple genes involved in starch and storage protein synthesis to activate their transcription. It also forms protein complexes with multiple transcription factors to synergistically regulate grain yield and quality. In addition, <i>OsMYB4</i> was directly activated by RISBZ1, a key regulator of storage reserve accumulation. Moreover, overexpression of <i>OsMYB4</i> resulted in larger grains and enhanced yield. Notably, natural variation analysis revealed that the beneficial haplotype <i>OsMYB4</i>-Hap1 is positively associated with increased grain weight and superior quality. In conclusion, our results demonstrate the regulatory role of <i>OsMYB4</i> for grain development, which provides valuable genetic resources for simultaneously improving grain yield and quality in rice.</p>

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The OsMYB4 transcription factor enables coordinated improvement of grain yield and quality in rice

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月7日

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  • Huan Xu,
  • Erchao Duan,
  • Qingkai Wang,
  • Rongbo Chen,
  • Chuanwei Gu,
  • Yipeng Zhang,
  • Wenjing Zhang,
  • Rushuang Zhang,
  • Rongxin Tao,
  • Yunpeng Zhang,
  • Wenting Lv,
  • Jiajia Chi,
  • Huimin Zhai,
  • Zhihao Qiao,
  • Zhuo Chen,
  • Xuan Teng,
  • Yunlu Tian,
  • Shijia Liu,
  • Xi Liu,
  • Ling Jiang,
  • Yuqiang Liu,
  • Yihua Wang,
  • Jianmin Wan

摘要

The endosperm is the predominant storage organ for starch and storage proteins in cereal grains, which also determines both grain yield and quality. Although key enzymes involved in the biosynthesis of starch and storage proteins have been identified, the underlying regulatory mechanisms remain largely obscure. In this study, we identified OsMYB4, a MYB transcription factor, specifically expressed in rice (Oryza sativa L.) endosperm. Loss-of-function of OsMYB4 led to increased grain chalkiness, accompanied by reduced amylose and protein contents as well as decreased grain yield. OsMYB4 directly binds to the promoters of multiple genes involved in starch and storage protein synthesis to activate their transcription. It also forms protein complexes with multiple transcription factors to synergistically regulate grain yield and quality. In addition, OsMYB4 was directly activated by RISBZ1, a key regulator of storage reserve accumulation. Moreover, overexpression of OsMYB4 resulted in larger grains and enhanced yield. Notably, natural variation analysis revealed that the beneficial haplotype OsMYB4-Hap1 is positively associated with increased grain weight and superior quality. In conclusion, our results demonstrate the regulatory role of OsMYB4 for grain development, which provides valuable genetic resources for simultaneously improving grain yield and quality in rice.