Scholarlink

<p>Regeneration can proceed through epimorphosis, involving regrowth of lost structures at the amputation site in a proximal-to-distal sequence, or through morphallaxis, which is characterized by extensive tissue remodeling with minimal cell proliferation. While the mechanisms underlying epimorphic regeneration are well studied, the spatiotemporal dynamics of morphallaxis remain less understood. Here we show, using time-course single-cell RNA sequencing (scRNA-seq) of regenerating Hydra tissue, that morphallaxis involves a temporal sequence in the re-emergence of terminal and subterminal cell identities, with transcriptional signatures associated with proximal structures appearing earlier than those of distal structures. We further find that Wnt signaling remains dynamically active at the amputation site throughout regeneration, with its composition changing over time, consistent with a transient regulatory state. Together, these findings provide a high-resolution view of dynamic cell state transitions and signaling activity during morphallactic regeneration and offer a framework for exploring how organizer function and axial patterning are re-established in <i>Hydra</i>.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Dynamic cell fate transitions during morphallactic regeneration

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年6月2日

查看全文
  • Clara Nuninger,
  • Panagiotis Papasaikas,
  • Sera L. Weevers,
  • Jacqueline Ferralli,
  • Sébastien Smallwood,
  • Charisios D. Tsiairis

摘要

Regeneration can proceed through epimorphosis, involving regrowth of lost structures at the amputation site in a proximal-to-distal sequence, or through morphallaxis, which is characterized by extensive tissue remodeling with minimal cell proliferation. While the mechanisms underlying epimorphic regeneration are well studied, the spatiotemporal dynamics of morphallaxis remain less understood. Here we show, using time-course single-cell RNA sequencing (scRNA-seq) of regenerating Hydra tissue, that morphallaxis involves a temporal sequence in the re-emergence of terminal and subterminal cell identities, with transcriptional signatures associated with proximal structures appearing earlier than those of distal structures. We further find that Wnt signaling remains dynamically active at the amputation site throughout regeneration, with its composition changing over time, consistent with a transient regulatory state. Together, these findings provide a high-resolution view of dynamic cell state transitions and signaling activity during morphallactic regeneration and offer a framework for exploring how organizer function and axial patterning are re-established in Hydra.