Scholarlink

<p>Conventional stimulated Raman scattering (SRS) microscopy requires time-consuming Raman-shift scanning to achieve chemical specificity, limiting its application in real-time biological studies. We present simultaneous dual-Raman-shift scanning-free SRS (SDRSRS) microscopy, which captures different distinct molecular vibrational signatures simultaneously without spectral scanning, achieving more than 10-fold faster imaging while maintaining high chemical specificity. The system uses birefringent crystals to create controlled optical path differences and orthogonal polarizations, enabling dual-phase modulation with custom dual-channel detection. The collinear balanced detection mode provides 11 dB signal-to-noise ratio improvement, and the system is compatible with other real-time imaging modalities. SDRSRS enables simultaneous CH₃ and CH₂ mapping to reveal lipid droplet dynamics in living cells, quantitatively distinguishes immunotherapy responses in liver cancer models, and provides rapid label-free classification of human colorectal cancer. This technique enables rapid and simultaneous dual vibrational contrast, providing chemically specific imaging for live metabolic monitoring, clinical pathology, and drug-response studies.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Simultaneous dual-Raman-shift scanning-free stimulated Raman scattering microscopy for label-free biomolecular imaging

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月19日

查看全文
  • Binglin Shen,
  • Zhi Zeng,
  • Haiyang Li,
  • Zongyi Yin,
  • Jiazi Yu,
  • Zhenglin Li,
  • Chao Zhang,
  • Yide Zhang,
  • Rui Hu,
  • Junle Qu,
  • Liwei Liu

摘要

Conventional stimulated Raman scattering (SRS) microscopy requires time-consuming Raman-shift scanning to achieve chemical specificity, limiting its application in real-time biological studies. We present simultaneous dual-Raman-shift scanning-free SRS (SDRSRS) microscopy, which captures different distinct molecular vibrational signatures simultaneously without spectral scanning, achieving more than 10-fold faster imaging while maintaining high chemical specificity. The system uses birefringent crystals to create controlled optical path differences and orthogonal polarizations, enabling dual-phase modulation with custom dual-channel detection. The collinear balanced detection mode provides 11 dB signal-to-noise ratio improvement, and the system is compatible with other real-time imaging modalities. SDRSRS enables simultaneous CH₃ and CH₂ mapping to reveal lipid droplet dynamics in living cells, quantitatively distinguishes immunotherapy responses in liver cancer models, and provides rapid label-free classification of human colorectal cancer. This technique enables rapid and simultaneous dual vibrational contrast, providing chemically specific imaging for live metabolic monitoring, clinical pathology, and drug-response studies.