Scholarlink

<p>All eukaryotes, including yeast, plants, animals and humans, possess linear chromosomes. The conserved eukaryotic telomere-telomerase systems, originated and evolved over 1 billion years, protect the chromosomal ends and regulate critical physiological functions through complex networks. In this study, we replace the endogenous eukaryotic telomeres in the single-chromosome yeast <i>Saccharomyces cerevisiae</i> with the prokaryotic telomere system TelN/<i>tos</i> from the <i>Escherichia</i> phage N15, which forms a closed hairpin structure, by interrupting the MRX/Sae2 pathway. The prokaryotic telomeres effectively protect linear chromosomal ends and prevent genetic instability. Through adaptive evolution, we identify yeast strains harboring additional mutations (<i>TEL1</i> and <i>CYR1</i>) that restore functional MRX/Sae2 activity, thereby improving host fitness and meiotic capacity. Interestingly, the two-associated TelN/<i>tos</i> telomeres position deeper into chromosomes and exhibit increased interactions with their adjacent regions. The successful replacement of a complex eukaryotic chromosomal telomere with a simple bacteriophage system demonstrates functional equivalence between these divergent systems, implying possible natural origins of such stochasticity (e.g., horizontal gene transfers). Furthermore, these engineered strains facilitate development of a <i>tos</i>-YAC system that enabled iterative assembly and stable maintenance of megabase-level heterogeneous DNA (1.23-2.77 Mb), providing a robust platform for large-scale DNA manipulation.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Engineering yeast chromosomal telomeres with a bacteriophage system

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月19日

查看全文
  • Weiqin Deng,
  • Yanyan Li,
  • Yangyang Shao,
  • Ronglin Zhao,
  • Fan Zhou,
  • Li Zhong,
  • Wang Liu,
  • Tao Wang,
  • Ting He,
  • Siying Zhang,
  • Huilin Chen,
  • Xin Man,
  • Can Hu,
  • Ping Fang,
  • Yongping Huang,
  • Xiangzhou Meng,
  • Jin-Qiu Zhou,
  • Zixin Deng,
  • Xiaoli Xue,
  • Zhongjun Qin

摘要

All eukaryotes, including yeast, plants, animals and humans, possess linear chromosomes. The conserved eukaryotic telomere-telomerase systems, originated and evolved over 1 billion years, protect the chromosomal ends and regulate critical physiological functions through complex networks. In this study, we replace the endogenous eukaryotic telomeres in the single-chromosome yeast Saccharomyces cerevisiae with the prokaryotic telomere system TelN/tos from the Escherichia phage N15, which forms a closed hairpin structure, by interrupting the MRX/Sae2 pathway. The prokaryotic telomeres effectively protect linear chromosomal ends and prevent genetic instability. Through adaptive evolution, we identify yeast strains harboring additional mutations (TEL1 and CYR1) that restore functional MRX/Sae2 activity, thereby improving host fitness and meiotic capacity. Interestingly, the two-associated TelN/tos telomeres position deeper into chromosomes and exhibit increased interactions with their adjacent regions. The successful replacement of a complex eukaryotic chromosomal telomere with a simple bacteriophage system demonstrates functional equivalence between these divergent systems, implying possible natural origins of such stochasticity (e.g., horizontal gene transfers). Furthermore, these engineered strains facilitate development of a tos-YAC system that enabled iterative assembly and stable maintenance of megabase-level heterogeneous DNA (1.23-2.77 Mb), providing a robust platform for large-scale DNA manipulation.