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<p>High-sensitivity glycomic analysis is essential for advancing both basic and translational biomedical research, yet remains methodologically challenging for limited-quantity samples, primarily due to complex workflows and reliance on specialized instrumentation. Here, we introduce solution-enhanced glycan reduction and permethylation (seGRAP), a streamlined, accessible, and high-sensitive method that enables glycan profiling from sub-nanogram protein inputs and picoliter-scale human plasma using widely available mass spectrometry (MS) platforms. seGRAP-MS demonstrates consistently robust and reproducible glycomic performance and enables comprehensive <i>N</i>-glycome characterization of human oocytes at single-cell resolution. Our single-oocyte analysis uncovers a highly conserved <i>N</i>-glycome across both individuals and developmental stages, revealing a previously uncharacterized layer of molecular stability in human reproduction. By significantly lowering technical and logistical barriers, seGRAP-MS redefines the benchmark for high-sensitive glycomics, expanding accessibility and empowering broader applications in fundamental biology, clinical research, and precision medicine.</p>

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High-sensitive glycomics using seGRAP-mass spectrometry uncovers a conserved N-glycome in single human oocytes

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月3日

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  • Nafisa Tursumamat,
  • Qiannan Liu,
  • Jihong Lu,
  • Shuhong Guo,
  • Shuangshuang Du,
  • Shengyang Liu,
  • Shuye Wu,
  • Heming Li,
  • Peng Wu,
  • Bin Li,
  • Juan Wei

摘要

High-sensitivity glycomic analysis is essential for advancing both basic and translational biomedical research, yet remains methodologically challenging for limited-quantity samples, primarily due to complex workflows and reliance on specialized instrumentation. Here, we introduce solution-enhanced glycan reduction and permethylation (seGRAP), a streamlined, accessible, and high-sensitive method that enables glycan profiling from sub-nanogram protein inputs and picoliter-scale human plasma using widely available mass spectrometry (MS) platforms. seGRAP-MS demonstrates consistently robust and reproducible glycomic performance and enables comprehensive N-glycome characterization of human oocytes at single-cell resolution. Our single-oocyte analysis uncovers a highly conserved N-glycome across both individuals and developmental stages, revealing a previously uncharacterized layer of molecular stability in human reproduction. By significantly lowering technical and logistical barriers, seGRAP-MS redefines the benchmark for high-sensitive glycomics, expanding accessibility and empowering broader applications in fundamental biology, clinical research, and precision medicine.