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<p>Detergent-free immunolabeling has been proven feasible for volumetric correlated light and electron microscopy (vCLEM) with the application of small immunoprobes, providing a reliable method to superimpose essential molecular information of cells onto their high-resolution electron microscopic images. However, the application of this method is restricted by the availability of suitable affinity reagents. Here we introduce CAptVE, a method using slow off-rate modified aptamers for cell fluorescence labeling on ultrastructurally reconstructable electron micrographs. CAptVE provides labeling for a wide range of biomarkers, offering a pathway to integrate molecular analysis into volumetric electron microscopic studies of tissues, including neural circuit investigations via connectomics.</p>

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Probing molecular diversity and ultrastructure of brain cells with fluorescent aptamers

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月27日

查看全文
  • Xiaotang Lu,
  • Yuelong Wu,
  • Peter H. Li,
  • Tao Fang,
  • Richard L. Schalek,
  • Yaxin Su,
  • Daniel R. Berger,
  • Jeffery D. Carter,
  • Sean Lynch,
  • Daniel J. Schneider,
  • Shashi Gupta,
  • Viren Jain,
  • Nebojsa Janjic,
  • Jeff W. Lichtman

摘要

Detergent-free immunolabeling has been proven feasible for volumetric correlated light and electron microscopy (vCLEM) with the application of small immunoprobes, providing a reliable method to superimpose essential molecular information of cells onto their high-resolution electron microscopic images. However, the application of this method is restricted by the availability of suitable affinity reagents. Here we introduce CAptVE, a method using slow off-rate modified aptamers for cell fluorescence labeling on ultrastructurally reconstructable electron micrographs. CAptVE provides labeling for a wide range of biomarkers, offering a pathway to integrate molecular analysis into volumetric electron microscopic studies of tissues, including neural circuit investigations via connectomics.