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<p>Current intravital imaging techniques for the mouse central nervous system (CNS) do not simultaneously provide micrometer-scale spatial resolution, whole-brain coverage, and sub-minute temporal resolution, limiting organ-wide interrogation of CNS fluid dynamics in vivo. Here, we introduce intravital synchrotron radiation-based hard X-ray micro computed tomography (SRµCT), a modality that enables dynamic whole-brain imaging at micrometer-scale spatial resolution in living mice. We performed intravital SRµCT of mouse CNS fluid spaces at three synchrotron radiation facilities, imaging both anesthetized free-breathing and mechanically ventilated animals, with and without retrospective cardiac gating. This approach achieves complete brain coverage with temporal resolution of up to 23 s and voxel sizes down to 6.3 µm, at an effective spatial resolution better than 20 µm, enabling time-resolved visualization of cerebrospinal fluid (CSF) contrast distribution and quantitative analysis of tissue motion across the entire brain. By combining micrometer-scale resolution, whole-organ field of view, and dynamic intravital imaging, SRµCT closes a long-standing methodological gap between optical microscopy and magnetic resonance imaging. Intravital SRµCT provides access to spatiotemporal information that cannot be obtained with existing techniques and establishes a framework for testing and integrating mechanistic models of CSF dynamics and solute transport at the scale of the whole brain.</p>

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In vivo imaging of central nervous system fluid spaces using synchrotron radiation-based micro computed tomography

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月2日

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  • Marta Girona Alarcón,
  • Willy Kuo,
  • Mattia Humbel,
  • Christine Tanner,
  • Luca Fardin,
  • Britta Bausch,
  • Yann Decker,
  • Irene Spera,
  • Griffin Rodgers,
  • Hans Deyhle,
  • Alberto Bravin,
  • Masato Hoshino,
  • Arash Panahifar,
  • Kentaro Uesugi,
  • Sergei Gasilov,
  • Petr Pleskač,
  • Yuansheng Zhang,
  • Diane de Zélicourt,
  • Amandine Brenna,
  • Ahmad Kamal Hamid,
  • Pooya Razzaghi Khamesi,
  • Britta Engelhardt,
  • Steven T. Proulx,
  • Bert Müller,
  • Vartan Kurtcuoglu

摘要

Current intravital imaging techniques for the mouse central nervous system (CNS) do not simultaneously provide micrometer-scale spatial resolution, whole-brain coverage, and sub-minute temporal resolution, limiting organ-wide interrogation of CNS fluid dynamics in vivo. Here, we introduce intravital synchrotron radiation-based hard X-ray micro computed tomography (SRµCT), a modality that enables dynamic whole-brain imaging at micrometer-scale spatial resolution in living mice. We performed intravital SRµCT of mouse CNS fluid spaces at three synchrotron radiation facilities, imaging both anesthetized free-breathing and mechanically ventilated animals, with and without retrospective cardiac gating. This approach achieves complete brain coverage with temporal resolution of up to 23 s and voxel sizes down to 6.3 µm, at an effective spatial resolution better than 20 µm, enabling time-resolved visualization of cerebrospinal fluid (CSF) contrast distribution and quantitative analysis of tissue motion across the entire brain. By combining micrometer-scale resolution, whole-organ field of view, and dynamic intravital imaging, SRµCT closes a long-standing methodological gap between optical microscopy and magnetic resonance imaging. Intravital SRµCT provides access to spatiotemporal information that cannot be obtained with existing techniques and establishes a framework for testing and integrating mechanistic models of CSF dynamics and solute transport at the scale of the whole brain.