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<p>Human heart development depends on tightly coordinated genetic programs and biomechanical cues, yet the underlying cell–microenvironment interactions remain poorly understood because the developing heart is difficult to study in utero and accurate experimental models are lacking. Recent advances in stem cell biology and three-dimensional (3D) bioprinting now allow the construction of human tissue analogues with defined structure and function. Here we show a perfusable 3D bioprinted model of the human embryonic heart tube composed of layered myocardium, cardiac jelly, and endocardium. Human induced pluripotent stem cell–derived cardiomyocytes and endothelial cells are cultured under controlled flow conditions, producing constructs with high cell viability, complete lumen endothelialization, progressive myocardial compaction, and coordinated tissue-level contraction. Single-cell transcriptomic analysis reveals that dynamic flow promotes cardiac maturation and lineage specification. This platform provides a human-relevant model to study early heart development, investigate congenital heart disease mechanisms, and evaluate emerging therapeutic strategies.</p>

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Modeling early human heart development using an iPSC-based 3D bioprinted model of embryonic heart tube

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月24日

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  • Linqi Jin,
  • Christian Park,
  • Sunder Neelakantan,
  • Shweta Karnik,
  • Arnab Dey,
  • Sarah Fineman,
  • Roshni Nandwani,
  • Carmen J. Gil,
  • Boeun Hwang,
  • Melissa A. Cadena,
  • Vani Sridhar,
  • Jing Wang,
  • Yuxiao Wu,
  • Connor J. Evans,
  • Lan Li,
  • Sarah Rezapourdamanab,
  • Mehdi Salar Amoli,
  • Martin L. Tomov,
  • Ryan K. Roeder,
  • Reza Avazmohammadi,
  • Lakshmi Prasad Dasi,
  • Hanjoong Jo,
  • Eric Weeks,
  • Holly D. Bauser-Heaton,
  • Sean M. Wu,
  • Vahid Serpooshan

摘要

Human heart development depends on tightly coordinated genetic programs and biomechanical cues, yet the underlying cell–microenvironment interactions remain poorly understood because the developing heart is difficult to study in utero and accurate experimental models are lacking. Recent advances in stem cell biology and three-dimensional (3D) bioprinting now allow the construction of human tissue analogues with defined structure and function. Here we show a perfusable 3D bioprinted model of the human embryonic heart tube composed of layered myocardium, cardiac jelly, and endocardium. Human induced pluripotent stem cell–derived cardiomyocytes and endothelial cells are cultured under controlled flow conditions, producing constructs with high cell viability, complete lumen endothelialization, progressive myocardial compaction, and coordinated tissue-level contraction. Single-cell transcriptomic analysis reveals that dynamic flow promotes cardiac maturation and lineage specification. This platform provides a human-relevant model to study early heart development, investigate congenital heart disease mechanisms, and evaluate emerging therapeutic strategies.