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<p>The mycotoxin deoxynivalenol (DON) poses severe threats to human and animal well-being globally. Enzymatic degradation is the most effective way to eliminate DON toxicity, yet no catalytic process for complete degradation of DON has been uncovered. Here, we show that a metabolic pathway initiated by C3-epimerization and C8-reduction is responsible for complete degradation of DON in the DON-metabolizing bacterium <i>Nocardioides</i> sp. S5-5. Two horizontally transferred aldo-keto reductase genes, <i>DONepi</i> and <i>DONrd</i>, have evolved to orchestrate C3-epimerization and C8-reduction respectively. Notably, the octameric-structured DONepi alone catalyzes C3-epimerization of DON by steering the rigid-body rotation of the transient 3-keto intermediate for stereoinverting reduction. Moreover, DONrd can catalyze the C8-reduction of DON and its C3-epimerized product 3-epi-DON simultaneously to form C8-hydroxyl products, which facilitates the further degradation by a potential oxidase and other putative enzymes. <i>DONepi</i> expression in transgenic plants confers resistance to DON, representing potential for controlling mycotoxin contamination pre- and postharvest.</p>

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Specialized aldo-keto reductases trigger complete degradation of mycotoxin deoxynivalenol

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年2月26日

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  • Weijie He,
  • Renyi Xiong,
  • Mengru Zheng,
  • Tiantian Zhang,
  • Yushan Zhang,
  • Qiang Wang,
  • Changxing Zhao,
  • Tao Huang,
  • Yike Liu,
  • Ye Tian,
  • Karim M. Tabl,
  • Xiaoming Mao,
  • Pan Li,
  • Guangwu Feng,
  • Xuechen Bai,
  • Qian Liu,
  • Wenhao Yan,
  • Yucai Liao,
  • Jingbo Zhang,
  • Ping Yin,
  • Aibo Wu

摘要

The mycotoxin deoxynivalenol (DON) poses severe threats to human and animal well-being globally. Enzymatic degradation is the most effective way to eliminate DON toxicity, yet no catalytic process for complete degradation of DON has been uncovered. Here, we show that a metabolic pathway initiated by C3-epimerization and C8-reduction is responsible for complete degradation of DON in the DON-metabolizing bacterium Nocardioides sp. S5-5. Two horizontally transferred aldo-keto reductase genes, DONepi and DONrd, have evolved to orchestrate C3-epimerization and C8-reduction respectively. Notably, the octameric-structured DONepi alone catalyzes C3-epimerization of DON by steering the rigid-body rotation of the transient 3-keto intermediate for stereoinverting reduction. Moreover, DONrd can catalyze the C8-reduction of DON and its C3-epimerized product 3-epi-DON simultaneously to form C8-hydroxyl products, which facilitates the further degradation by a potential oxidase and other putative enzymes. DONepi expression in transgenic plants confers resistance to DON, representing potential for controlling mycotoxin contamination pre- and postharvest.