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<p>Securinega alkaloids possess a distinctive tetracyclic scaffold and are pharmaceutically relevant, yet their biosynthesis in <i>Flueggea suffruticosa</i> remains unresolved. Here, we combine chemical logic with stable-isotope-labeled feeding experiments and single-cell transcriptomics to elucidate the core biosynthetic pathway. We identify a reductase, <i>FsMS</i>, that reduces premenisdaurilide to menisdaurilide, which then conjugates with 1-piperideine to generate the [2.2.2]-bicyclic neosecurinanes, (–)-virosine A and (–)-virosine B. We also identify two sulfotransferases, <i>FsNSST1</i> and <i>FsNSST2</i>, that <i>O</i>-sulfate these neosecurinanes; the resulting sulfate esters undergo a spontaneous 1,2-amine shift to yield the [3.2.1]-bicyclic securinanes, allosecurinine and securinine. This transformation reveals an unexpected biosynthetic role of sulfotransferases, not as conventional tailoring enzymes, but as key mediators of scaffold remodeling. The newly identified biosynthetic genes are co-expressed within a specific vasculature-associated cell type, enabling prioritization of additional pathway candidates. These findings highlight the power of chemically guided single-cell transcriptomics in unraveling complex biosynthetic pathways.</p>

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Chemically guided single-cell transcriptomics reveals sulfotransferase-mediated scaffold remodeling in securinine biosynthesis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月23日

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  • Sungjun Choung,
  • Gyumin Kang,
  • Taein Kim,
  • Seoyoung Kim,
  • Hyejung Yun,
  • Yeojin Hwang,
  • Hyeonjin Kim,
  • Hangah Lim,
  • Kwanyoung Moon,
  • Sunkyu Han,
  • Sang-Gyu Kim

摘要

Securinega alkaloids possess a distinctive tetracyclic scaffold and are pharmaceutically relevant, yet their biosynthesis in Flueggea suffruticosa remains unresolved. Here, we combine chemical logic with stable-isotope-labeled feeding experiments and single-cell transcriptomics to elucidate the core biosynthetic pathway. We identify a reductase, FsMS, that reduces premenisdaurilide to menisdaurilide, which then conjugates with 1-piperideine to generate the [2.2.2]-bicyclic neosecurinanes, (–)-virosine A and (–)-virosine B. We also identify two sulfotransferases, FsNSST1 and FsNSST2, that O-sulfate these neosecurinanes; the resulting sulfate esters undergo a spontaneous 1,2-amine shift to yield the [3.2.1]-bicyclic securinanes, allosecurinine and securinine. This transformation reveals an unexpected biosynthetic role of sulfotransferases, not as conventional tailoring enzymes, but as key mediators of scaffold remodeling. The newly identified biosynthetic genes are co-expressed within a specific vasculature-associated cell type, enabling prioritization of additional pathway candidates. These findings highlight the power of chemically guided single-cell transcriptomics in unraveling complex biosynthetic pathways.