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<p>The transmission of the most virulent human malaria parasite, <i>Plasmodium falciparum</i>, relies on its survival in the contrasting environments of the human host and mosquito vector. One of the most fascinating adaptations to this lifestyle is the specific silencing of individual rDNA genes in the human host that are de-repressed following host-to-vector transmission. In this study, we define the epigenetic signatures of rRNA transcription and find that rDNA silencing relies on aerobic glycolysis, the sole energy-generating pathway in the human host. We show that disruption of NAD<sup>+</sup> regeneration during lactate fermentation promotes rDNA de-repression and identify the sirtuin histone deacetylase Sir2a as the mediator between fluctuating NAD<sup>+</sup> levels and a functional transcriptional outcome. Hence, rDNA activation appears to be coupled to the metabolic state of the parasite as it transitions from aerobic glycolysis to mitochondrial respiration during host-to-vector transmission.</p>

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A metabolism-chromatin axis promotes differential ribosomal RNA transcription in the human malaria parasite

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月19日

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  • Justine E. Couble,
  • Tiziano Vignolini,
  • Grégory Doré,
  • Selina Mussgnug,
  • Bérangère Lombard,
  • Michael Richard,
  • Damarys Loew,
  • Michael Büttner,
  • Rafael Dueñas-Sánchez,
  • Gernot Poschet,
  • Jessica M. Bryant,
  • Sebastian Baumgarten

摘要

The transmission of the most virulent human malaria parasite, Plasmodium falciparum, relies on its survival in the contrasting environments of the human host and mosquito vector. One of the most fascinating adaptations to this lifestyle is the specific silencing of individual rDNA genes in the human host that are de-repressed following host-to-vector transmission. In this study, we define the epigenetic signatures of rRNA transcription and find that rDNA silencing relies on aerobic glycolysis, the sole energy-generating pathway in the human host. We show that disruption of NAD+ regeneration during lactate fermentation promotes rDNA de-repression and identify the sirtuin histone deacetylase Sir2a as the mediator between fluctuating NAD+ levels and a functional transcriptional outcome. Hence, rDNA activation appears to be coupled to the metabolic state of the parasite as it transitions from aerobic glycolysis to mitochondrial respiration during host-to-vector transmission.