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<p>Loss-of-function mutations in <i>AKAP11</i> (a protein kinase A (PKA)-binding protein) greatly increase the risk of bipolar disorder and schizophrenia. To determine the neurobiological functions of AKAP11, we conduct multi-omic and neurobiological analyses of <i>Akap11</i> mutant mouse brains. We find that AKAP11 is a key regulator of PKA proteostasis in the brain whose loss leads to dramatically increased levels of PKA subunits and phosphorylated PKA substrates, especially in synapses. <i>Akap11</i> mutant mice show extensive transcriptomic changes throughout the brain, including prominent decreases in synapse-related genes sets. Gene expression is highly impacted in spiny projection neurons of the striatum, a brain region implicated in motivation, cognition and psychotic disorders. Real-time measurements of PKA activity reveal elevated basal PKA activity in the&#xa0;striatum of <i>Akap11</i><sup><i>-/-</i></sup> mice, with exaggerated additional response to dopamine receptor antagonists. Behaviorally, <i>Akap11</i> mutant mice show abnormally prolonged locomotor response to amphetamine, deficits in associative learning and contextual discrimination, as well as depression-like behaviors. Our study connects molecular changes to circuit dysfunction and behavioral disturbance in a genetically valid animal model of psychotic disorder.</p>

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Elevated synaptic PKA activity and abnormal striatal dopamine signaling in Akap11 mutant mice, a genetic model of schizophrenia and bipolar disorder

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月28日

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  • Bryan J. Song,
  • Yang Ge,
  • Ally Nicolella,
  • Min Jee Kwon,
  • Bart Lodder,
  • Kevin Bonanno,
  • Antia Valle-Tojeiro,
  • Nolan D. Hartley,
  • Kira Perzel Mandell,
  • John Adeleye,
  • Deeksha Misri,
  • Chuhan Geng,
  • Sahana Natarajan,
  • Inès Picard,
  • Nate Shepard,
  • Alyssa Hall,
  • Jiawen Tian,
  • Sameer Aryal,
  • Zohreh Farsi,
  • Xiao-Man Liu,
  • Nader Morshed,
  • Naeem M. Nadaf,
  • Horia Pribiag,
  • Sean K. Simmons,
  • D. R. Mani,
  • Beth Stevens,
  • Prabhat S. Kunwar,
  • Zhanyan Fu,
  • Evan Z. Macosko,
  • Joshua Z. Levin,
  • Bernardo L. Sabatini,
  • Steven A. Carr,
  • Borislav Dejanovic,
  • Hasmik Keshishian,
  • Adam J. Granger,
  • Morgan Sheng

摘要

Loss-of-function mutations in AKAP11 (a protein kinase A (PKA)-binding protein) greatly increase the risk of bipolar disorder and schizophrenia. To determine the neurobiological functions of AKAP11, we conduct multi-omic and neurobiological analyses of Akap11 mutant mouse brains. We find that AKAP11 is a key regulator of PKA proteostasis in the brain whose loss leads to dramatically increased levels of PKA subunits and phosphorylated PKA substrates, especially in synapses. Akap11 mutant mice show extensive transcriptomic changes throughout the brain, including prominent decreases in synapse-related genes sets. Gene expression is highly impacted in spiny projection neurons of the striatum, a brain region implicated in motivation, cognition and psychotic disorders. Real-time measurements of PKA activity reveal elevated basal PKA activity in the striatum of Akap11-/- mice, with exaggerated additional response to dopamine receptor antagonists. Behaviorally, Akap11 mutant mice show abnormally prolonged locomotor response to amphetamine, deficits in associative learning and contextual discrimination, as well as depression-like behaviors. Our study connects molecular changes to circuit dysfunction and behavioral disturbance in a genetically valid animal model of psychotic disorder.