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<p>The inversion of C3 stereochemistry in monoterpenoid indole alkaloids (MIAs), derived from the central precursor strictosidine (3<i>S</i>), is essential for producing pharmacologically important 3 <i>R</i> MIAs and spirooxindoles such as reserpine. While early MIA biosynthesis preserves the 3<i>S</i> configuration, the mechanism underlying C3 inversion has remained unresolved. Here, we identify and biochemically characterize a conserved oxidase-reductase pair in Gentianales: heteroyohimbine/yohimbine/corynanthe C3-oxidase (HYC3O) and C3-reductase (HYC3R), which together invert the 3<i>S</i> stereochemistry to 3 <i>R</i> across diverse substrates. HYC3O and HYC3R are encoded within biosynthetic gene clusters in <i>Rauvolfia tetraphylla</i> and <i>Catharanthus roseus</i>, homologous to a geissoschizine synthase (GS) cluster also uncovered. Comparative genomics indicate that the GS cluster originated at the base of Gentianales (~135 Mya), coinciding with the evolution of the strictosidine synthase cluster, whereas the reserpine cluster arose later. These findings uncover the genomic and biochemical basis of key events driving MIA diversification beyond canonical vinblastine and ajmaline pathways.</p>

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Ancient gene clusters govern the initiation of monoterpenoid indole alkaloid biosynthesis and C3 stereochemistry inversion

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月25日

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  • Jaewook Hwang,
  • Jonathan Kirshner,
  • Daniel André Ramey Deschênes,
  • Matthew Bailey Richardson,
  • Steven J. Fleck,
  • Scott Galeung Alexander Mann,
  • Jun Guo,
  • Jacob Owen Perley,
  • Mohammadamin Shahsavarani,
  • Jorge Jonathan Oswaldo Garza-Garcia,
  • Alyssa Dawn Seveck,
  • Savannah Sadie Doiron,
  • Zhan Mai,
  • Stephen Nelson Silliphant,
  • Sarah Anne Englehart,
  • Barry A. Blight,
  • Larry Calhoun,
  • Di Gao,
  • Jiazhang Lian,
  • Ghislain Deslongchamps,
  • Victor A. Albert,
  • Yang Qu

摘要

The inversion of C3 stereochemistry in monoterpenoid indole alkaloids (MIAs), derived from the central precursor strictosidine (3S), is essential for producing pharmacologically important 3 R MIAs and spirooxindoles such as reserpine. While early MIA biosynthesis preserves the 3S configuration, the mechanism underlying C3 inversion has remained unresolved. Here, we identify and biochemically characterize a conserved oxidase-reductase pair in Gentianales: heteroyohimbine/yohimbine/corynanthe C3-oxidase (HYC3O) and C3-reductase (HYC3R), which together invert the 3S stereochemistry to 3 R across diverse substrates. HYC3O and HYC3R are encoded within biosynthetic gene clusters in Rauvolfia tetraphylla and Catharanthus roseus, homologous to a geissoschizine synthase (GS) cluster also uncovered. Comparative genomics indicate that the GS cluster originated at the base of Gentianales (~135 Mya), coinciding with the evolution of the strictosidine synthase cluster, whereas the reserpine cluster arose later. These findings uncover the genomic and biochemical basis of key events driving MIA diversification beyond canonical vinblastine and ajmaline pathways.