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<p>Tobacco is a significant industrial crop, serving as a model for plant science and a promising specie for the production of proteins and small molecules. However, system biology studies of tobacco under natural field cultivation conditions remain scarce. Here, we construct a genome-scale metabolic regulatory network through integration of dynamic transcriptomic and metabolomic profiles from field-grown tobacco leaves across two ecologically distinct regions. We map 25,984 genes and 633 metabolites into 3.17 million regulatory pairs using multi-algorithm integration. This network reveals three pivotal transcriptional hubs, including <i>NtMYB28</i> (promoting hydroxycinnamic acids synthesis by modifying <i>Nt4CL2</i> and <i>NtPAL2</i> expression), <i>NtERF167</i> (amplifying lipid synthesis via <i>NtLACS2</i> activation) and <i>NtCYC</i> (driving aroma production through <i>NtLOX2</i> induction). These transcriptional hubs achieve substantial yield improvements of target metabolites by rewiring metabolic flux. The present work provides a systems-level atlas of tobacco metabolic regulation and may help to guide metabolic engineering.</p>

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Multi-omics analyses reveal regulatory networks underpinning metabolite biosynthesis in Nicotiana tabacum

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月24日

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  • Jiaming Li,
  • Qinggang Liao,
  • Huina Zhou,
  • Risheng Hu,
  • Yangyang Li,
  • Zhengrong Hu,
  • Bei Yu,
  • Pingping Liu,
  • Qingxia Zheng,
  • Wenxuan Pu,
  • Song Sheng,
  • Yongjun Liu,
  • Shaolong Wu,
  • Tianbo Liu,
  • Qinzhi Xiao,
  • Shuhui Duan,
  • Junping Gao,
  • Xiaoxu Li,
  • Shuaibin Wang,
  • Hanqian Xiao,
  • Zhicheng Zhou,
  • Zhongshan Lu,
  • Jiashuo Yang,
  • Jianbin Yan

摘要

Tobacco is a significant industrial crop, serving as a model for plant science and a promising specie for the production of proteins and small molecules. However, system biology studies of tobacco under natural field cultivation conditions remain scarce. Here, we construct a genome-scale metabolic regulatory network through integration of dynamic transcriptomic and metabolomic profiles from field-grown tobacco leaves across two ecologically distinct regions. We map 25,984 genes and 633 metabolites into 3.17 million regulatory pairs using multi-algorithm integration. This network reveals three pivotal transcriptional hubs, including NtMYB28 (promoting hydroxycinnamic acids synthesis by modifying Nt4CL2 and NtPAL2 expression), NtERF167 (amplifying lipid synthesis via NtLACS2 activation) and NtCYC (driving aroma production through NtLOX2 induction). These transcriptional hubs achieve substantial yield improvements of target metabolites by rewiring metabolic flux. The present work provides a systems-level atlas of tobacco metabolic regulation and may help to guide metabolic engineering.