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<p>Current methods for detecting <i>Mycobacterium tuberculosis (M.tb)</i> in centralized medical facilities are a bottleneck in TB surveillance, particularly in resource-constrained regions. In response, we present a groundbreaking portable bio-tool, the lab-in-a-cartridge (LIC) system, designed for on-site detection of lipoarabinomannan (LAM) in trace amounts within the urine. The innovative design combines pumpless liquid handling and magnetic force-based enrichment with horseradish peroxidase polymer amplification to precisely quantify low biomarker levels. Employing a tetramethylbenzidine-based colorimetric reaction, the LIC enables semi-quantitative LAM detection. This LIC incorporates all necessary reagents, achieving a detection threshold of as low as 0.01 pg/mL in pooled urine samples within 40 minutes. The LIC distinguishes TB patients in clinical urine samples with 92% sensitivity and 88% specificity. This pioneering device not only sets an improved standard for detecting low LAM concentrations but also holds the potential to realize a decentralized diagnosis of TB.</p>

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Lab-in-a-cartridge for real-time detection of tuberculosis via precise measurement of urinary lipoarabinomannan

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月21日

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  • Woong Heo,
  • Qingyang Wang,
  • Seoyeon Choi,
  • Joonchul Shin,
  • Seong Jun Park,
  • Sunyoung Park,
  • Dong-Uk Kim,
  • Jungho Kim,
  • Young Ae Kang,
  • Hyo-Il Jung

摘要

Current methods for detecting Mycobacterium tuberculosis (M.tb) in centralized medical facilities are a bottleneck in TB surveillance, particularly in resource-constrained regions. In response, we present a groundbreaking portable bio-tool, the lab-in-a-cartridge (LIC) system, designed for on-site detection of lipoarabinomannan (LAM) in trace amounts within the urine. The innovative design combines pumpless liquid handling and magnetic force-based enrichment with horseradish peroxidase polymer amplification to precisely quantify low biomarker levels. Employing a tetramethylbenzidine-based colorimetric reaction, the LIC enables semi-quantitative LAM detection. This LIC incorporates all necessary reagents, achieving a detection threshold of as low as 0.01 pg/mL in pooled urine samples within 40 minutes. The LIC distinguishes TB patients in clinical urine samples with 92% sensitivity and 88% specificity. This pioneering device not only sets an improved standard for detecting low LAM concentrations but also holds the potential to realize a decentralized diagnosis of TB.