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<p>Powdery mildew is a devastating disease that affects wheat yield and quality globally. Here, we identify a powdery mildew resistance locus <i>MlIW39</i> from wild emmer wheat through map-based cloning, mutagenesis, and stable genetic transformation. Unlike many other cloned <i>Pm</i> genes, the <i>MlIW39</i>-mediated resistance is conferred by the combined effect of two complementary nucleotide-binding and leucine-rich repeat (NLR) genes, encoding a canonical coiled-coil (CC) type NLR protein (<i>MlIW39-R1</i>) and an atypical NLR protein (<i>MlIW39-R2</i>) with an unknown domain (CC-like), respectively. Overexpression of the NLR pair induces cell death in <i>Nicotiana benthamiana</i>, whereas <i>MlIW39-R1</i> or <i>MlIW39-R2</i> alone does not. The MlIW39-R1 and MlIW39-R2 proteins physically interact with each other. <i>MlIW39-R1</i> and <i>MlIW39-R2</i> likely originate independently and become neighborly located during evolution. Our findings shed light on the significance of NLR pairs in plant immunity and can facilitate wheat disease-resistance breeding using the developed <i>MlIW39</i> introgression lines and functional marker.</p>

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Two complementary NLRs from wild emmer wheat confer powdery mildew resistance

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月10日

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  • Zuhuan Yang,
  • Nannan Liu,
  • Xiaoming Xie,
  • Wenxin Wei,
  • Yuhan Bai,
  • Junna Sun,
  • Wei Pan,
  • Jiatian Yang,
  • Weidong Wang,
  • Xiaodong Xie,
  • Muhammad Saqlain,
  • Houyang Kang,
  • Baoyun Li,
  • Zhaorong Hu,
  • Jinying Gou,
  • Weilong Guo,
  • Susheng Song,
  • Jun Ma,
  • Tzion Fahima,
  • Qixin Sun,
  • Lina Qiu,
  • Yinghui Li,
  • Chaojie Xie

摘要

Powdery mildew is a devastating disease that affects wheat yield and quality globally. Here, we identify a powdery mildew resistance locus MlIW39 from wild emmer wheat through map-based cloning, mutagenesis, and stable genetic transformation. Unlike many other cloned Pm genes, the MlIW39-mediated resistance is conferred by the combined effect of two complementary nucleotide-binding and leucine-rich repeat (NLR) genes, encoding a canonical coiled-coil (CC) type NLR protein (MlIW39-R1) and an atypical NLR protein (MlIW39-R2) with an unknown domain (CC-like), respectively. Overexpression of the NLR pair induces cell death in Nicotiana benthamiana, whereas MlIW39-R1 or MlIW39-R2 alone does not. The MlIW39-R1 and MlIW39-R2 proteins physically interact with each other. MlIW39-R1 and MlIW39-R2 likely originate independently and become neighborly located during evolution. Our findings shed light on the significance of NLR pairs in plant immunity and can facilitate wheat disease-resistance breeding using the developed MlIW39 introgression lines and functional marker.