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<p>Phosphorus (P) is an essential macro-nutrient for plant growth and development. It is preferentially taken as inorganic phosphate (Pi). Plants have evolved elaborate mechanisms to adapt to low Pi (LP) stress through activating Pi-starvation responses. H<sub>2</sub>O<sub>2</sub> is an important signal molecule involved in plant adaptation to diverse environmental stresses. However, whether H<sub>2</sub>O<sub>2</sub> plays a role in Pi-starvation responses remains unknown. Here, we reveal that H<sub>2</sub>O<sub>2</sub> produced by the respiratory burst oxidase homologs OsRBOH-D/H facilitates phosphate uptake and utilization under LP conditions in rice. Mechanistically LP-induced H<sub>2</sub>O<sub>2</sub> promotes the oxidization of the key phosphate signaling transcription factor PHOSPHATE STARVATION RESPONSE2 (OsPHR2) at its Cys377 residue to trigger its oligomerization, sequence-specific DNA binding ability, and nuclear translocation, thereby activating Pi-starvation responses to adapt to LP conditions. Additionally, our molecular and biochemical data revealed that <i>OsRBOH-D</i> is a direct target gene of OsPHR2. Thus, OsPHR2 and OsRBOH-D form a positive feedback regulatory loop in which H<sub>2</sub>O<sub>2</sub> acts as a second messenger to amplify the Pi-starvation response.</p>

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H2O2-mediated oxidation of PHOSPHATE STARVATION RESPONSE2 promotes adaptation to low phosphate in rice

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月1日

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  • Funing Meng,
  • Dan Xiang,
  • Ziqi Bu,
  • Rongbin Lin,
  • Xinyang Sun,
  • Jiming Xu,
  • Yunrong Wu,
  • Yu Liu,
  • Zhongchang Wu,
  • Xiaorong Mo,
  • Javier Paz-Ares,
  • Chuanzao Mao

摘要

Phosphorus (P) is an essential macro-nutrient for plant growth and development. It is preferentially taken as inorganic phosphate (Pi). Plants have evolved elaborate mechanisms to adapt to low Pi (LP) stress through activating Pi-starvation responses. H2O2 is an important signal molecule involved in plant adaptation to diverse environmental stresses. However, whether H2O2 plays a role in Pi-starvation responses remains unknown. Here, we reveal that H2O2 produced by the respiratory burst oxidase homologs OsRBOH-D/H facilitates phosphate uptake and utilization under LP conditions in rice. Mechanistically LP-induced H2O2 promotes the oxidization of the key phosphate signaling transcription factor PHOSPHATE STARVATION RESPONSE2 (OsPHR2) at its Cys377 residue to trigger its oligomerization, sequence-specific DNA binding ability, and nuclear translocation, thereby activating Pi-starvation responses to adapt to LP conditions. Additionally, our molecular and biochemical data revealed that OsRBOH-D is a direct target gene of OsPHR2. Thus, OsPHR2 and OsRBOH-D form a positive feedback regulatory loop in which H2O2 acts as a second messenger to amplify the Pi-starvation response.