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<p>Allocation of resources in the costly proteome reflects trade-offs between cellular functions. For example, proteome composition of <i>Escherichia coli</i> is significantly regulated by growth rate. An increasing anabolic, especially ribosomal, proteome fraction correlates with a decreasing catabolic proteome fraction at faster growth, which then leads to changes in catabolism. Our systems-level studies of the thermophilic acetogen <i>Thermoanaerobacter kivui</i> when growth rate is varied over two orders of magnitude revealed a different strategy: proteome allocation is only partially controlled by growth rate, and metabolic rates are primarily controlled posttranslationally. At slower growth, ribosome numbers are controlled by rRNA concentrations with an excess of ribosomal proteins. Composition of the catabolic proteome is uncoupled from catabolic rates as indicated by flux analysis. This study adds to the understanding of acetogenic Clostridia, which are of interest for biotechnological processes in a carbon-neutral economy, and points to a complex landscape of microbial ecophysiological strategies.</p>

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Non-canonical resource allocation in heterotrophically growing Thermoanaerobacter kivui

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年9月26日

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  • Franziska Maria Mueller,
  • Albert Leopold Müller,
  • Wenyu Gu,
  • Farshad Abdollah-Nia,
  • Jiawei Sun,
  • Jenna Kim Ahn,
  • Kerwyn Casey Huang,
  • James R. Williamson,
  • Alfred Michael Spormann

摘要

Allocation of resources in the costly proteome reflects trade-offs between cellular functions. For example, proteome composition of Escherichia coli is significantly regulated by growth rate. An increasing anabolic, especially ribosomal, proteome fraction correlates with a decreasing catabolic proteome fraction at faster growth, which then leads to changes in catabolism. Our systems-level studies of the thermophilic acetogen Thermoanaerobacter kivui when growth rate is varied over two orders of magnitude revealed a different strategy: proteome allocation is only partially controlled by growth rate, and metabolic rates are primarily controlled posttranslationally. At slower growth, ribosome numbers are controlled by rRNA concentrations with an excess of ribosomal proteins. Composition of the catabolic proteome is uncoupled from catabolic rates as indicated by flux analysis. This study adds to the understanding of acetogenic Clostridia, which are of interest for biotechnological processes in a carbon-neutral economy, and points to a complex landscape of microbial ecophysiological strategies.