Scholarlink

<p>Flowering at the appropriate time is one of the critical traits to determine regional adaptation and yield of soybeans. Although several genes in regulating flowering time have been identified in soybeans, the epigenetic regulators are still largely unknown. Here, we identify an early flowering mutant (<i>ef1</i>) and clone the causal gene as <i>lysine-specific demethylase like 2</i> (<i>GmLDL2</i>). Enzymatic activity assay shows that GmLDL2 has demethylase activity towards H3K4me1 and H3K4me2. Integrative ChIP-seq and RNA-seq analyses demonstrate that GmLDL2 directly inhibits the expression of <i>GmFER</i>, an ortholog of <i>Arabidopsis FERONIA</i>. Genetic analysis reveals that <i>GmLDL2</i> regulates soybean flowering in a <i>GmFER-</i>dependent manner. Interestingly, both GmLDL2<sup>H1</sup> and GmLDL2<sup>H2</sup> haploids display distinct demethylase activity and likely facilitate the adaptation of soybeans to low- and high-latitude regions, respectively. Together, our findings reveal the GmLDL2-GmFER module in regulating soybean flowering and provide useful information for manipulating the regional adaptability of soybeans.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Natural variation in histone demethylase GmLDL2 confers flowering time divergence for geographical expansion in soybean

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年7月1日

查看全文
  • Mengshi Liu,
  • Yuan Fang,
  • Jiacan Jiang,
  • Huaizhen Chen,
  • Bing Yang,
  • Xiaomin Xu,
  • Guoqiang Liu,
  • Chen Ling,
  • Zhicheng Dong,
  • Cunyi Yang,
  • Yingxiang Wang

摘要

Flowering at the appropriate time is one of the critical traits to determine regional adaptation and yield of soybeans. Although several genes in regulating flowering time have been identified in soybeans, the epigenetic regulators are still largely unknown. Here, we identify an early flowering mutant (ef1) and clone the causal gene as lysine-specific demethylase like 2 (GmLDL2). Enzymatic activity assay shows that GmLDL2 has demethylase activity towards H3K4me1 and H3K4me2. Integrative ChIP-seq and RNA-seq analyses demonstrate that GmLDL2 directly inhibits the expression of GmFER, an ortholog of Arabidopsis FERONIA. Genetic analysis reveals that GmLDL2 regulates soybean flowering in a GmFER-dependent manner. Interestingly, both GmLDL2H1 and GmLDL2H2 haploids display distinct demethylase activity and likely facilitate the adaptation of soybeans to low- and high-latitude regions, respectively. Together, our findings reveal the GmLDL2-GmFER module in regulating soybean flowering and provide useful information for manipulating the regional adaptability of soybeans.