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<p>Lung tissue-resident memory T cells (T<sub>RM</sub>) are critical for the local control of respiratory tract infections caused by influenza A viruses (IAV). Here we compare T<sub>RM</sub> populations induced by intranasal adenoviral vector vaccines encoding hemagglutinin and nucleoprotein (NP) with those induced by an H1N1 infection in BALB/c mice. While vaccine-induced T<sub>RM</sub> express high levels of CD103 and persist longer in the lung parenchyma, short-lived, H1N1-induced T<sub>RM</sub> have a transcriptome associated with higher cytotoxic potential and distinct transcriptional profile as shown by single-cell RNA sequencing. In both the vaccine and H1N1 groups, NP-specific CD8<sup>+</sup> T cells expand during heterologous influenza virus infection and protect the mice from disease. Meanwhile, lung inflammation in response to an infection with unrelated respiratory syncytial virus do not influence the fate of pre-existing T<sub>RM</sub>. Our preclinical work thus confirms that inflammatory conditions in the tissue shape the phenotypic and functional characteristics of T<sub>RM</sub> to serve relevant informations for optimizing mucosal vaccines.</p>

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Inflammatory conditions shape phenotypic and functional characteristics of lung-resident memory T cells in mice

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月16日

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  • Anna Schmidt,
  • Jana Fuchs,
  • Mark Dedden,
  • Katharina Kocher,
  • Christine Schülein,
  • Julian Hübner,
  • Ana Vieira Antão,
  • Pascal Irrgang,
  • Friederike Oltmanns,
  • Vera Viherlehto,
  • Natascha Leicht,
  • Ralf Joachim Rieker,
  • Carol Geppert,
  • Uwe Appelt,
  • Sebastian Zundler,
  • Kilian Schober,
  • Dennis Lapuente,
  • Matthias Tenbusch

摘要

Lung tissue-resident memory T cells (TRM) are critical for the local control of respiratory tract infections caused by influenza A viruses (IAV). Here we compare TRM populations induced by intranasal adenoviral vector vaccines encoding hemagglutinin and nucleoprotein (NP) with those induced by an H1N1 infection in BALB/c mice. While vaccine-induced TRM express high levels of CD103 and persist longer in the lung parenchyma, short-lived, H1N1-induced TRM have a transcriptome associated with higher cytotoxic potential and distinct transcriptional profile as shown by single-cell RNA sequencing. In both the vaccine and H1N1 groups, NP-specific CD8+ T cells expand during heterologous influenza virus infection and protect the mice from disease. Meanwhile, lung inflammation in response to an infection with unrelated respiratory syncytial virus do not influence the fate of pre-existing TRM. Our preclinical work thus confirms that inflammatory conditions in the tissue shape the phenotypic and functional characteristics of TRM to serve relevant informations for optimizing mucosal vaccines.