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<p>Small nucleolar RNAs are non-coding transcripts that guide chemical modifications of RNA substrates and modulate gene expression at the epigenetic and post-transcriptional levels. However, the extent of their regulatory potential and the underlying molecular mechanisms remain poorly understood. Here, we identify a conserved, previously unannotated intronic C/D-box snoRNA, termed <i>snR107</i>, hosted in the fission yeast long non-coding RNA <i>mamRNA</i> and carrying two independent cellular functions. On the one hand, <i>snR107</i> guides site-specific 25S rRNA 2’-O-methylation and promotes pre-rRNA processing and 60S subunit biogenesis. On the other hand, <i>snR107</i> associates with the gametogenic RNA-binding proteins Mmi1 and Mei2, mediating their reciprocal inhibition and restricting meiotic gene expression during sexual differentiation. Both functions require distinct <i>cis</i>-motifs within <i>snR107</i>, including a conserved 2’-O-methylation guiding sequence. Together, our results position <i>snR107</i> as a dual regulator of rRNA modification and gametogenesis effectors, expanding our vision on the non-canonical functions exerted by snoRNAs in cell fate decisions.</p>

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A bifunctional snoRNA with separable activities in guiding rRNA 2’-O-methylation and scaffolding gametogenesis effectors

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月5日

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  • Estelle Leroy,
  • Drice Challal,
  • Stéphane Pelletier,
  • Coralie Goncalves,
  • Alexandra Menant,
  • Virginie Marchand,
  • Yan Jaszczyszyn,
  • Erwin van Dijk,
  • Delphine Naquin,
  • Jessica Andreani,
  • Yuri Motorin,
  • Benoit Palancade,
  • Mathieu Rougemaille

摘要

Small nucleolar RNAs are non-coding transcripts that guide chemical modifications of RNA substrates and modulate gene expression at the epigenetic and post-transcriptional levels. However, the extent of their regulatory potential and the underlying molecular mechanisms remain poorly understood. Here, we identify a conserved, previously unannotated intronic C/D-box snoRNA, termed snR107, hosted in the fission yeast long non-coding RNA mamRNA and carrying two independent cellular functions. On the one hand, snR107 guides site-specific 25S rRNA 2’-O-methylation and promotes pre-rRNA processing and 60S subunit biogenesis. On the other hand, snR107 associates with the gametogenic RNA-binding proteins Mmi1 and Mei2, mediating their reciprocal inhibition and restricting meiotic gene expression during sexual differentiation. Both functions require distinct cis-motifs within snR107, including a conserved 2’-O-methylation guiding sequence. Together, our results position snR107 as a dual regulator of rRNA modification and gametogenesis effectors, expanding our vision on the non-canonical functions exerted by snoRNAs in cell fate decisions.