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<p>Starch is a primary food ingredient and industrial feedstock. Low-carbon microbial manufacturing offers a carbon-neutral/negative arable land-independent strategy for starch production. Here, we reconfigure the oleaginous yeast as a starch-rich micro-grain producer by rewiring the starch biosynthesis and gluconeogenesis pathways and regulating cell morphology. With the CO<sub>2</sub> electro-synthesized acetate as the substrate, the strain accumulates starch 47.18% of dry cell weight. The optimized system renders spatial-temporal starch productivity (243.7 g/m<sup>2</sup>/d) approximately 50-fold higher than crop cultivation and volumetric productivity (160.83 mg/L/h) over other microbial systems by an order of magnitude. We demonstrate tunable starch composition and starch-protein ratios via strain and process engineering. The engineered artificial strains adopt a cellular resources reallocation strategy to ensure high-level starch production in micro-grain and could facilitate a highly efficient straw/cellulose-to-starch conversion. This work elucidates starch biosynthesis machinery and establishes a superior-to-nature platform for customizable starch synthesis, advancing low-carbon nutritional manufacturing.</p>

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Reprogramming yeast metabolism for customized starch-rich micro-grain through low-carbon microbial manufacturing

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年3月21日

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  • Zhihui Shi,
  • Zhaoyu Xu,
  • Weihe Rong,
  • Hongbing Sun,
  • Hongyi Zhou,
  • Qianqian Yuan,
  • Aixuan Xiao,
  • Hongfei Ma,
  • Tao Cai,
  • Guokun Wang,
  • Yanhe Ma

摘要

Starch is a primary food ingredient and industrial feedstock. Low-carbon microbial manufacturing offers a carbon-neutral/negative arable land-independent strategy for starch production. Here, we reconfigure the oleaginous yeast as a starch-rich micro-grain producer by rewiring the starch biosynthesis and gluconeogenesis pathways and regulating cell morphology. With the CO2 electro-synthesized acetate as the substrate, the strain accumulates starch 47.18% of dry cell weight. The optimized system renders spatial-temporal starch productivity (243.7 g/m2/d) approximately 50-fold higher than crop cultivation and volumetric productivity (160.83 mg/L/h) over other microbial systems by an order of magnitude. We demonstrate tunable starch composition and starch-protein ratios via strain and process engineering. The engineered artificial strains adopt a cellular resources reallocation strategy to ensure high-level starch production in micro-grain and could facilitate a highly efficient straw/cellulose-to-starch conversion. This work elucidates starch biosynthesis machinery and establishes a superior-to-nature platform for customizable starch synthesis, advancing low-carbon nutritional manufacturing.