Scholarlink

<p>Fusarium crown rot (FCR) is a serious underlying disease to threaten wheat yield and quality recently. Here, we identify a catalase antioxidant enzyme (<i>TaCAT2</i>) through genome wide association study (GWAS) and whole-exome sequencing (WES) in two nested bi-parental populations. We verify the function of <i>TaCAT2</i> regulating wheat FCR resistance by genetic transformation. Moreover, we screen a sucrose non-fermenting-1-related protein kinase alpha subunit (TaSnRK1α) interacting with TaCAT2, and subsequently find that TaSnRK1α phosphorylates TaCAT2. We next identify an FCR-resistance haplotype TaCAT2<sup>Ser214</sup>, and confirm that Ser214 of TaCAT2 is a key phosphorylation site for TaSnRK1α. We also find that TaSnRK1α results in higher protein accumulation in TaCAT2<sup>Ser214</sup> than in TaCAT2<sup>Thr214</sup>, which possibly contribute to scavenging ROS (reactive oxygen species) in TaCAT2<sup>Ser214</sup> wheat plants. Furthermore, the function of <i>TaSnRK1α</i> regulating FCR resistance is verified by genetic transformation. Taken together, we propose a TaSnRK1α-TaCAT2 model to mediate FCR resistance by scavenging the ROS in wheat plants.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A TaSnRK1α-TaCAT2 model mediates resistance to Fusarium crown rot by scavenging ROS in common wheat

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年3月15日

查看全文
  • Xia Yang,
  • Leilei Zhang,
  • Jiajie Wei,
  • Lexin Liu,
  • Di Liu,
  • Xiangning Yan,
  • Minjie Yuan,
  • Lingran Zhang,
  • Ning Zhang,
  • Yan Ren,
  • Feng Chen

摘要

Fusarium crown rot (FCR) is a serious underlying disease to threaten wheat yield and quality recently. Here, we identify a catalase antioxidant enzyme (TaCAT2) through genome wide association study (GWAS) and whole-exome sequencing (WES) in two nested bi-parental populations. We verify the function of TaCAT2 regulating wheat FCR resistance by genetic transformation. Moreover, we screen a sucrose non-fermenting-1-related protein kinase alpha subunit (TaSnRK1α) interacting with TaCAT2, and subsequently find that TaSnRK1α phosphorylates TaCAT2. We next identify an FCR-resistance haplotype TaCAT2Ser214, and confirm that Ser214 of TaCAT2 is a key phosphorylation site for TaSnRK1α. We also find that TaSnRK1α results in higher protein accumulation in TaCAT2Ser214 than in TaCAT2Thr214, which possibly contribute to scavenging ROS (reactive oxygen species) in TaCAT2Ser214 wheat plants. Furthermore, the function of TaSnRK1α regulating FCR resistance is verified by genetic transformation. Taken together, we propose a TaSnRK1α-TaCAT2 model to mediate FCR resistance by scavenging the ROS in wheat plants.