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<p>Improvement of cold tolerance at the booting stage (CTB) in rice is a key strategy for cultivation in high-altitude and high-latitude regions. Here, we identify <i>CTB3</i> gene, encoding a calmodulin-binding transcriptional activator that positively regulates cold tolerance at the booting stage in <i>japonica</i> rice. Two indels (57-bp and 284-bp) in the <i>CTB3</i> promoter confer a differential transcriptional response to cold between the <i>japonica</i> and <i>indica</i> subspecies. OsTCP19 suppresses <i>CTB3</i> expression by binding to these indels, negatively regulating cold tolerance. CTB3 activates the expression of <i>TREHALOSE-6-PHOSPHATE PHOSPHATASE1</i> (<i>OsTPP1</i>), reducing trehalose 6-phosphate (Tre6P) levels, which increases sugar accumulation in panicles and improves cold tolerance. Additionally, favorable alleles of <i>OsTCP19</i> and <i>CTB3</i> are selected in <i>japonica</i> rice for cold adaptation. These findings highlight the important role of <i>CTB3</i> in cold adaptation and its potential for improving cold tolerance in rice breeding.</p>

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Natural variation of indels in the CTB3 promoter confers cold tolerance in japonica rice

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年2月13日

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  • Jin Li,
  • Haifeng Guo,
  • Qijin Lou,
  • Yawen Zeng,
  • Zhenhua Guo,
  • Penghui Xu,
  • Yunsong Gu,
  • Shilei Gao,
  • Bingxia Xu,
  • Shichen Han,
  • Runbin Su,
  • Andong Zou,
  • Wei Ye,
  • Meng Zhang,
  • Yingxiu Li,
  • Xingming Sun,
  • Zhanying Zhang,
  • Hongliang Zhang,
  • Wendong Ma,
  • Chao Chen,
  • Zichao Li,
  • Jinjie Li

摘要

Improvement of cold tolerance at the booting stage (CTB) in rice is a key strategy for cultivation in high-altitude and high-latitude regions. Here, we identify CTB3 gene, encoding a calmodulin-binding transcriptional activator that positively regulates cold tolerance at the booting stage in japonica rice. Two indels (57-bp and 284-bp) in the CTB3 promoter confer a differential transcriptional response to cold between the japonica and indica subspecies. OsTCP19 suppresses CTB3 expression by binding to these indels, negatively regulating cold tolerance. CTB3 activates the expression of TREHALOSE-6-PHOSPHATE PHOSPHATASE1 (OsTPP1), reducing trehalose 6-phosphate (Tre6P) levels, which increases sugar accumulation in panicles and improves cold tolerance. Additionally, favorable alleles of OsTCP19 and CTB3 are selected in japonica rice for cold adaptation. These findings highlight the important role of CTB3 in cold adaptation and its potential for improving cold tolerance in rice breeding.