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<p>Photosynthesis harvests solar energy to convert CO<sub>2</sub> into chemicals, offering a potential solution to reduce atmospheric CO<sub>2</sub>. However, integrating photosynthesis into non-photosynthetic microbes to utilize one-carbon substrates is challenging. Here, a photosynthesis system is reconstructed in <i>E. coli</i>, by integrating light and dark reaction to synthesize bioproducts from one-carbon substrates. A light reaction is reconstructed using the photosystem of photosynthetic bacteria, increasing ATP and NADH contents by 337.9% and 383.7%, respectively. A dark reaction is constructed by designing CO<sub>2</sub> fixation pathway to synthesize pyruvate. By assembling the light and dark reaction, a photosynthesis system is established and further programmed by installing an energy adapter, enabling the production of acetone, malate, and α-ketoglutarate, with a negative carbon footprint of −0.84 ~ −0.23 kgCO<sub>2</sub>e/kg product. Furthermore, light-driven one-carbon trophic growth of <i>E. coli</i> is achieved with a doubling time of 19.86 h. This photosynthesis system provides a green and sustainable approach to enhance one-carbon substrates utilization in the future.</p>

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A new-to-nature photosynthesis system enhances utilization of one-carbon substrates in Escherichia coli

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年1月2日

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  • Tian Tong,
  • Xiulai Chen,
  • Kexin Tang,
  • Wanrong Ma,
  • Cong Gao,
  • Wei Song,
  • Jing Wu,
  • Xiaoling Wang,
  • Gao-Qiang Liu,
  • Liming Liu

摘要

Photosynthesis harvests solar energy to convert CO2 into chemicals, offering a potential solution to reduce atmospheric CO2. However, integrating photosynthesis into non-photosynthetic microbes to utilize one-carbon substrates is challenging. Here, a photosynthesis system is reconstructed in E. coli, by integrating light and dark reaction to synthesize bioproducts from one-carbon substrates. A light reaction is reconstructed using the photosystem of photosynthetic bacteria, increasing ATP and NADH contents by 337.9% and 383.7%, respectively. A dark reaction is constructed by designing CO2 fixation pathway to synthesize pyruvate. By assembling the light and dark reaction, a photosynthesis system is established and further programmed by installing an energy adapter, enabling the production of acetone, malate, and α-ketoglutarate, with a negative carbon footprint of −0.84 ~ −0.23 kgCO2e/kg product. Furthermore, light-driven one-carbon trophic growth of E. coli is achieved with a doubling time of 19.86 h. This photosynthesis system provides a green and sustainable approach to enhance one-carbon substrates utilization in the future.