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<p>A novel actinomycete strain, cg5<sup>T</sup>, was isolated from the rhizosphere soil of <i>Cyclosorus parasiticus</i>, collected from Xiangtan City, Hunan province, China. Strain cg5<sup>T</sup> contained <i>meso</i>-diaminopimelic acid as a diagnostic amino acid. Whole-cell sugars of strain cg5<sup>T</sup> included arabinose, galactose, ribose, and rhamnose. The chemotaxonomic properties of this strain are typical of members of the genus <i>Amycolatopsis</i>. The genomic DNA G + C content of strain cg5<sup>T</sup>, whose genome size is approximately 9.7 Mb, was 68.5%. The full-length 16S rRNA sequence analysis indicated that this strain was a member of the genus <i>Amycolatopsis</i> and shared the highest sequence similarity to <i>Amycolatopsis xylanica</i> CPCC 202699<sup>T</sup> (99.23%). Phylogenetic trees based on 16S rRNA gene and genome sequences revealed that strain cg5<sup>T</sup> was most closely related to <i>A. xylanica</i> CPCC 202699<sup>T</sup>. However, the ANIm and dDDH values between these two strains were 92.23% and 45.80%, respectively, far lower than the 96.6% ANIm and 70% dDDH cut-off points recommended for delineating species. Moreover, phenotypic and chemotaxonomic characteristics further confirm that strain cg5<sup>T</sup> represents a new species of the genus <i>Amycolatopsis</i>, for which the name <i>Amycolatopsis cyclosori</i> sp. nov. is proposed. The type strain is cg5<sup>T</sup> (=MCCC 1K09227<sup>T</sup> = KCTC 59391<sup>T</sup>).</p>

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Amycolatopsis cyclosori sp. nov., isolated from the rhizosphere soil of Cyclosorus parasiticus

  • 期刊论文

  • 发表日期：

    2025年8月26日

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  • Ya-Zi Chen,
  • Rong-Jian Gao,
  • Ming-Yue Li,
  • Yan Xiao,
  • Jian Gao

摘要

A novel actinomycete strain, cg5T, was isolated from the rhizosphere soil of Cyclosorus parasiticus, collected from Xiangtan City, Hunan province, China. Strain cg5T contained meso-diaminopimelic acid as a diagnostic amino acid. Whole-cell sugars of strain cg5T included arabinose, galactose, ribose, and rhamnose. The chemotaxonomic properties of this strain are typical of members of the genus Amycolatopsis. The genomic DNA G + C content of strain cg5T, whose genome size is approximately 9.7 Mb, was 68.5%. The full-length 16S rRNA sequence analysis indicated that this strain was a member of the genus Amycolatopsis and shared the highest sequence similarity to Amycolatopsis xylanica CPCC 202699T (99.23%). Phylogenetic trees based on 16S rRNA gene and genome sequences revealed that strain cg5T was most closely related to A. xylanica CPCC 202699T. However, the ANIm and dDDH values between these two strains were 92.23% and 45.80%, respectively, far lower than the 96.6% ANIm and 70% dDDH cut-off points recommended for delineating species. Moreover, phenotypic and chemotaxonomic characteristics further confirm that strain cg5T represents a new species of the genus Amycolatopsis, for which the name Amycolatopsis cyclosori sp. nov. is proposed. The type strain is cg5T (=MCCC 1K09227T = KCTC 59391T).