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<p>A novel actinobacterium strain, designated HUAS ZL42<sup>T</sup>, was isolated from the leaves of <i>Cynara scolymus</i>. Its taxonomic position was characterized using a polyphasic method. Strain HUAS ZL42<sup>T</sup> produced spiral spore chains consisting of rod-shaped or ovoid spores with smooth surfaces on Gause’s synthetic No.1 medium. The cell wall of strain HUAS ZL42<sup>T</sup> contained <span>ll</span>-diaminopimelic acid as a diagnostic amino acid. Whole-cell hydrolysates contained galactose and mannose. The predominant cellular fatty acids (&gt;10.0%) of strain HUAS ZL42<sup>T</sup> were iso-C<sub>14:0</sub>, anteiso-C<sub>15:0</sub> and iso-C<sub>16:0</sub>. The menaquinones were MK-9 (H<sub>6</sub>), MK-9 (H<sub>4</sub>) and MK-9 (H<sub>8</sub>). The 16S rRNA gene sequence analysis showed that strain HUAS ZL42<sup>T</sup> exhibited the highest similarities to <i>Streptomyces cyslabdanicus</i> K04-0144<sup>T</sup> (98.6%), <i>Streptomyces cinnabarigriseus</i> JS360<sup>T</sup> (98.6%), <i>Streptomyces cyaneus</i> CGMCC 4.1671<sup>T</sup> (98.4%) and <i>Streptomyces corchorusii</i> DSM 40340<sup>T</sup> (98.4%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HUAS ZL42<sup>T</sup> formed an independent subclade. Phylogenetic analysis based on five housekeeping gene sequences demonstrated that strain HUAS ZL42<sup>T</sup> was most closely related to <i>S. cyaneus</i> CGMCC 4.1671<sup>T</sup>. However, the ANIm and dDDH between strain HUAS ZL42<sup>T</sup> and <i>S. cyaneus</i> CGMCC 4.1671<sup>T</sup> were 87.17% and 28.90%, respectively, far less than 96.7% and 70% cut-off points recommended for delineating <i>Streptomyces</i> species, suggesting that strain HUAS ZL42<sup>T</sup> was a novel <i>Streptomyces</i> species. On the basis of phenotypic and genotypic differentiation from tested strains, it is proposed that strain HUAS ZL42<sup>T</sup> represents a novel species of the genus <i>Streptomyces</i>, named <i>Streptomyces secundicynarae</i> sp. nov. The type strain is HUAS ZL42<sup>T</sup> (=MCCC 1K09444<sup>T</sup> = JCM 37325<sup>T</sup>).</p>

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Streptomyces secundicynarae sp. nov., a novel actinomycete isolated from the leaves of Cynara scolymus

  • 期刊论文

  • 发表日期：

    2025年7月29日

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  • Yu Song,
  • Ting Tang,
  • Ping Mo,
  • Zhilong Wu,
  • Fengying Liu,
  • Aihua Deng

摘要

A novel actinobacterium strain, designated HUAS ZL42T, was isolated from the leaves of Cynara scolymus. Its taxonomic position was characterized using a polyphasic method. Strain HUAS ZL42T produced spiral spore chains consisting of rod-shaped or ovoid spores with smooth surfaces on Gause’s synthetic No.1 medium. The cell wall of strain HUAS ZL42T contained ll-diaminopimelic acid as a diagnostic amino acid. Whole-cell hydrolysates contained galactose and mannose. The predominant cellular fatty acids (>10.0%) of strain HUAS ZL42T were iso-C14:0, anteiso-C15:0 and iso-C16:0. The menaquinones were MK-9 (H6), MK-9 (H4) and MK-9 (H8). The 16S rRNA gene sequence analysis showed that strain HUAS ZL42T exhibited the highest similarities to Streptomyces cyslabdanicus K04-0144T (98.6%), Streptomyces cinnabarigriseus JS360T (98.6%), Streptomyces cyaneus CGMCC 4.1671T (98.4%) and Streptomyces corchorusii DSM 40340T (98.4%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HUAS ZL42T formed an independent subclade. Phylogenetic analysis based on five housekeeping gene sequences demonstrated that strain HUAS ZL42T was most closely related to S. cyaneus CGMCC 4.1671T. However, the ANIm and dDDH between strain HUAS ZL42T and S. cyaneus CGMCC 4.1671T were 87.17% and 28.90%, respectively, far less than 96.7% and 70% cut-off points recommended for delineating Streptomyces species, suggesting that strain HUAS ZL42T was a novel Streptomyces species. On the basis of phenotypic and genotypic differentiation from tested strains, it is proposed that strain HUAS ZL42T represents a novel species of the genus Streptomyces, named Streptomyces secundicynarae sp. nov. The type strain is HUAS ZL42T (=MCCC 1K09444T = JCM 37325T).