<p>Cytoskeletal filaments may be actively driven by motor proteins and subjected to thermal fluctuations. Active filaments are self-propelling polymers inspired by cytoskeletal filaments and exhibit a rich spectrum of behaviors that are absent in their passive counterparts, posing challenges and opportunities for polymer physics. Microtubule-based active filaments under constraints show movements reminiscent of biological motions, arising from the interplay between filament mechanics and motor activity and mechanics. However, the significant variability in experimentally determined microtubule bending rigidities complicates the interpretation and prediction of their behaviors. Here, using computer simulations of overdamped mechanics, we investigate how the bending rigidity of microtubules influences their behaviors when the leading ends are pinned and subsequently released from the pinning. By systematically varying the preset bending rigidities, we found that distinct behaviors emerge depending on the bending rigidity, which are visible manifestations of the mechanical properties of microtubules. These differences originated from the local dynamics near the pinned ends of the microtubules. This study suggests a novel methodology based on active filament dynamics for probing the mechanical properties of microtubules, where conventional methodologies face challenges in terms of accuracy and throughput. In addition, this study provides insight into the reliable operation of active filament-based devices.</p>