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<p>This article presents the results of the synthesis of biological macromolecules, namely, single-cell protein (SCP) and degradable polyhydroxyalkanoates (PHAs), by a new strain of <i>Cupriavidus necator</i> B-15081 with high lipase activity on waste fish oil (WFO). Bacteria were cultivated in flasks on medium containing 20.0 g/L WFO obtained from <i>Sprattus sprattus</i> and <i>Scomber scombrus</i> waste under different concentrations of the nitrogen source (urea) to modify the C/N ratio in the medium and the direction of constructive cell metabolism. On a medium with a urea concentration of 1.5 g/L, the bacterial biomass concentration reached 7.2 ± 0.3 g/L, the intracellular protein content was 50.7%, and the amino acid composition was complete with a full set of amino acids, including essential ones. In a slowly growing culture under nitrogen deficiency, the total biomass concentration reached 12.0 ± 0.9 g/L, and the intracellular content of PHAs was 86.4 ± 5.9%. Bacteria synthesized P(3HB) homopolymers that exhibited a reduced degree of crystallinity (49–50%). Moreover, the completeness of WFO use by bacteria exceeded 80%. According to the studied parameters, the strain <i>C. necator</i> B-15081 is a promising producer of target products on a new renewable substrate.</p>

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New strain Cupriavidus necator B-15081—a producer of single-cell protein and degradable polyhydroxyalkanoates on waste fish oil

  • 期刊论文

  • 发表日期：

    2026年4月17日

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  • Natalia Zhila,
  • Kristina Sapozhnikova,
  • Evgeniy Kiselev,
  • Anzhelika Kolmakova,
  • Tatiana Volova

摘要

This article presents the results of the synthesis of biological macromolecules, namely, single-cell protein (SCP) and degradable polyhydroxyalkanoates (PHAs), by a new strain of Cupriavidus necator B-15081 with high lipase activity on waste fish oil (WFO). Bacteria were cultivated in flasks on medium containing 20.0 g/L WFO obtained from Sprattus sprattus and Scomber scombrus waste under different concentrations of the nitrogen source (urea) to modify the C/N ratio in the medium and the direction of constructive cell metabolism. On a medium with a urea concentration of 1.5 g/L, the bacterial biomass concentration reached 7.2 ± 0.3 g/L, the intracellular protein content was 50.7%, and the amino acid composition was complete with a full set of amino acids, including essential ones. In a slowly growing culture under nitrogen deficiency, the total biomass concentration reached 12.0 ± 0.9 g/L, and the intracellular content of PHAs was 86.4 ± 5.9%. Bacteria synthesized P(3HB) homopolymers that exhibited a reduced degree of crystallinity (49–50%). Moreover, the completeness of WFO use by bacteria exceeded 80%. According to the studied parameters, the strain C. necator B-15081 is a promising producer of target products on a new renewable substrate.