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Background <p>Colorectal cancer liver metastasis (CRLM) remains a major challenge in oncology, with the tumor microenvironment playing a crucial role in disease progression. This study investigates the function of the Tripartite Motif Containing 26 (TRIM26) in the CRLM microenvironment, focusing on its regulation of tumor-associated macrophage (TAM) polarization and its implications for metastatic growth.</p> Methods <p>Using established mouse CRLM models, we characterized TAM phenotypes using flow cytometry and immunohistochemistry. In vitro co-culture experiments evaluated the effects of <i>Trim26</i>-deficient bone marrow-derived macrophages (BMDMs) on tumor cell behavior. Western blotting and luciferase reporter assays were employed to elucidate the underlying molecular mechanisms.</p> Results <p><i>Trim26</i> knockout mice exhibited significantly reduced liver metastasis and an increased proportion of M1-like TAMs. <i>Trim26</i>-deficient BMDMs suppressed tumor cell migration and proliferation. TRIM26 modulates macrophage polarization by inhibiting the NF-κB signaling pathway. Specifically, TRIM26 interacts with TRAF2 through its PRY domain and inhibits the K63-linked ubiquitination of TRAF2, thereby attenuating NF-κB pathway activation. Furthermore, clinical CRLM samples revealed a negative correlation between TRIM26 expression and M1-like TAM infiltration.</p> Conclusion <p>We identified TRIM26 as a potential therapeutic target for CRLM, providing novel insights into tumor-stromal microenvironment interactions and offering new strategies to improve patient outcomes.</p>

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TRIM26 deficiency potentially suppresses colorectal cancer liver metastasis through NF-κB-mediated M1-like tumor-associated macrophage polarization

  • 期刊论文

  • 发表日期：

    2025年6月9日

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  • Wei Zhong,
  • Yuqi Zhang,
  • Weiwei Wang,
  • Zile Shao,
  • Ziyi Xu,
  • Gongye Zhang,
  • Zhixing Gao,
  • Zhizhong Zheng,
  • Yayu Zhang,
  • Houyu Chen,
  • Gang Song

摘要

Background

Colorectal cancer liver metastasis (CRLM) remains a major challenge in oncology, with the tumor microenvironment playing a crucial role in disease progression. This study investigates the function of the Tripartite Motif Containing 26 (TRIM26) in the CRLM microenvironment, focusing on its regulation of tumor-associated macrophage (TAM) polarization and its implications for metastatic growth.

Methods

Using established mouse CRLM models, we characterized TAM phenotypes using flow cytometry and immunohistochemistry. In vitro co-culture experiments evaluated the effects of Trim26-deficient bone marrow-derived macrophages (BMDMs) on tumor cell behavior. Western blotting and luciferase reporter assays were employed to elucidate the underlying molecular mechanisms.

Results

Trim26 knockout mice exhibited significantly reduced liver metastasis and an increased proportion of M1-like TAMs. Trim26-deficient BMDMs suppressed tumor cell migration and proliferation. TRIM26 modulates macrophage polarization by inhibiting the NF-κB signaling pathway. Specifically, TRIM26 interacts with TRAF2 through its PRY domain and inhibits the K63-linked ubiquitination of TRAF2, thereby attenuating NF-κB pathway activation. Furthermore, clinical CRLM samples revealed a negative correlation between TRIM26 expression and M1-like TAM infiltration.

Conclusion

We identified TRIM26 as a potential therapeutic target for CRLM, providing novel insights into tumor-stromal microenvironment interactions and offering new strategies to improve patient outcomes.