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<p>This study aimed to compare measurements of the renin-angiotensin-aldosterone system (RAAS) components by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and chemiluminescent immunoassay (CLIA) in hypertensive patients with different gender, position and renal function. EDTA plasma from 100 hypertensive patients who completed postural test were included. Aldosterone (PAC), renin (PRC, using Autobio CLIA microparticles kit; DRC, using LIAISON Direct Renin CLIA kit), angiotensin I and II (AngI and AngII), 18-hydroxycorticosterone (18-OHB) and 18-hydroxycortisol (18-OHF) were measured. Median PAC<sub>CLIA</sub> was 46.0% higher (<i>P</i> &lt; 0.01) than PAC<sub>LC-MS/MS</sub>. Median PAC<sub>CLIA</sub>, 18-OHB<sub>LC-MS/MS</sub> and 18-OHF<sub>LC-MS/MS</sub> were higher (<i>P</i> &lt; 0.01) in patients with renal dysfunction compared with patients with normal renal function, whereas median PAC<sub>LC-MS/MS</sub> did not show a significant difference between the two groups. Both median PRC<sub>CLIA</sub> and DRC<sub>CLIA</sub> were higher (<i>P</i> &lt; 0.01) in male patients compared with female patients. PAC<sub>LC-MS/MS</sub>, PAC<sub>CLIA</sub>, PRC<sub>CLIA</sub> and DRC<sub>CLIA</sub> all showed good consistency in response to an assumption of upright posture. Close (<i>P</i> &lt; 0.01) correlations were observed between PAC<sub>CLIA</sub> and PAC<sub>LC-MS/MS</sub>, between PRC<sub>CLIA</sub> and DRC<sub>CLIA</sub>, between AngII<sub>LC-MS/MS</sub> and PRC<sub>CLIA</sub>, and between AngII<sub>LC-MS/MS</sub> and DRC<sub>CLIA</sub>. LC-MS/MS is a reliable assay for measurement of the RAAS components in hypertensive patients under different pathophysiological conditions.</p>

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Comparisons of renin-angiotensin-aldosterone levels measured by chemical luminescence immunoassay and liquid chromatography-tandem mass spectrometry in hypertensive patients under different pathophysiological conditions

  • 期刊论文

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  • 发表日期：

    2025年10月3日

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  • Ting-Ting Xu,
  • Yi-Gui Tang,
  • En-Jun Xu,
  • Xuan-Xuan Wang,
  • Yu-Ping Yang,
  • Zeng Guo

摘要

This study aimed to compare measurements of the renin-angiotensin-aldosterone system (RAAS) components by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and chemiluminescent immunoassay (CLIA) in hypertensive patients with different gender, position and renal function. EDTA plasma from 100 hypertensive patients who completed postural test were included. Aldosterone (PAC), renin (PRC, using Autobio CLIA microparticles kit; DRC, using LIAISON Direct Renin CLIA kit), angiotensin I and II (AngI and AngII), 18-hydroxycorticosterone (18-OHB) and 18-hydroxycortisol (18-OHF) were measured. Median PACCLIA was 46.0% higher (P < 0.01) than PACLC-MS/MS. Median PACCLIA, 18-OHBLC-MS/MS and 18-OHFLC-MS/MS were higher (P < 0.01) in patients with renal dysfunction compared with patients with normal renal function, whereas median PACLC-MS/MS did not show a significant difference between the two groups. Both median PRCCLIA and DRCCLIA were higher (P < 0.01) in male patients compared with female patients. PACLC-MS/MS, PACCLIA, PRCCLIA and DRCCLIA all showed good consistency in response to an assumption of upright posture. Close (P < 0.01) correlations were observed between PACCLIA and PACLC-MS/MS, between PRCCLIA and DRCCLIA, between AngIILC-MS/MS and PRCCLIA, and between AngIILC-MS/MS and DRCCLIA. LC-MS/MS is a reliable assay for measurement of the RAAS components in hypertensive patients under different pathophysiological conditions.